Characterization of Light-Enhanced Respiration in Cyanobacteria.
Characterization of Light-Enhanced Respiration in Cyanobacteria.
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DOI：
10.3390/ijms22010342
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发表时间：
2020-12-31
影响因子：
5.6
通讯作者：
Miyake C
中科院分区：
文献类型：
--
作者：
Shimakawa G;Kohara A;Miyake C
在真核藻类中，光照后呼吸O2的吸收增强，这被称为光增强呼吸（LER）。它可能是由光合作用过程中产生的呼吸底物的增加刺激的CO2同化和功能，在真核细胞的光保持代谢和氧化还原稳态，基于细胞质，叶绿体和线粒体之间的相互作用。在这里，我们首先表征LER在光合作用的原核蓝藻，其中呼吸和光合作用在一个细胞中共享其代谢和电子传递链。从生理分析，蓝细菌集胞藻属PCC 6803执行LER，类似于真核藻类，其显示出与净光合O2释放速率相当的能力。虽然呼吸和光合作用的电子传递共享链间，LER是解耦的光合电子传递。突变体分析表明，LER的动机是由光合CO2同化直接提供的底物，而不是由糖原。此外，光依赖性激活的LER观察，即使与外源性添加的葡萄糖，这意味着除了底物量的LER的调节机制。最后，我们讨论了生理意义的大容量的LER在蓝藻和真核藻类相比，在植物中，通常表现出较少的LER。
In eukaryotic algae, respiratory O2 uptake is enhanced after illumination, which is called light-enhanced respiration (LER). It is likely stimulated by an increase in respiratory substrates produced during photosynthetic CO2 assimilation and function in keeping the metabolic and redox homeostasis in the light in eukaryotic cells, based on the interactions among the cytosol, chloroplasts, and mitochondria. Here, we first characterize LER in photosynthetic prokaryote cyanobacteria, in which respiration and photosynthesis share their metabolisms and electron transport chains in one cell. From the physiological analysis, the cyanobacterium Synechocystis sp. PCC 6803 performs LER, similar to eukaryotic algae, which shows a capacity comparable to the net photosynthetic O2 evolution rate. Although the respiratory and photosynthetic electron transports share the interchain, LER was uncoupled from photosynthetic electron transport. Mutant analyses demonstrated that LER is motivated by the substrates directly provided by photosynthetic CO2 assimilation, but not by glycogen. Further, the light-dependent activation of LER was observed even with exogenously added glucose, implying a regulatory mechanism for LER in addition to the substrate amounts. Finally, we discuss the physiological significance of the large capacity of LER in cyanobacteria and eukaryotic algae compared to those in plants that normally show less LER.
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影响因子：
5.2
作者：
Benson PJ;Purcell-Meyerink D;Hocart CH;Truong TT;James GO;Rourke L;Djordjevic MA;Blackburn SI;Price GD
通讯作者：
Price GD
DOI：
10.1098/rstb.2016.0379
发表时间：
2017-09-26
影响因子：
6.3
作者：
Feilke, Kathleen;Ajlani, Ghada;Krieger-Liszkay, Anja
通讯作者：
Krieger-Liszkay, Anja
影响因子：
4.1
作者：
Massoz, Simon;Larosa, Veronique;Cardol, Pierre
通讯作者：
Cardol, Pierre
影响因子：
9.2
作者：
Helman, Y;Tchernov, D;Kaplan, A
通讯作者：
Kaplan, A
影响因子：
7.4
作者：
Ermakova, Maria;Huokko, Tuomas;Allahverdiyeva, Yagut
通讯作者：
Allahverdiyeva, Yagut