AFLP linkage group assignment to the chromosomes of Allium cepa L-via monosomic addition lines
AFLP linkage group assignment to the chromosomes of Allium cepa L-via monosomic addition lines
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DOI：
10.1007/s001220050062
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发表时间：
2000-02-01
影响因子：
5.4
通讯作者：
Kik, C
中科院分区：
文献类型：
--
作者：
van Heusden, AW;Shigyo, M;Kik, C
两套完整的葱属植物。- A. cepa单体附加系（2n=2x+1=17）和基于A. cepa和A. royleiSteam对8个A. cepa连锁群的定位研究。连锁群可以被分配到单个的物理染色体上。A. cepa和A. fistulosum使186个扩增片段长度多态性（AFLP（TM）标记）的鉴定成为可能。cepa和A. fistulosum与10种不同的引物组合。利用单体附加系，研究了标记在八条染色体上的分布。cepa是可以确定的。在这186个AFLP标记中，有51个标记在A. roylei，因此在作图研究中用作标记（A. cepa x A. roylei cross）。因此，这51个AFLP标记可用于8个A. cepa连锁群在物理染色体上的定位研究中确定。还包括7个同工酶和3个CAPS标记。其中两个连锁群必须分开，因为它们包括对应于不同染色体的两组标记。共20个（约。10%）。cepa特异性AFLP标记扩增在一个以上类型的单体附加系，表明非连锁重复。利用共显性同工酶和CAPS分子标记，对来源于A. cepa或A. roylei。
Two complete sets of Allium fistulosum L. - A. cepa monosomic addition lines (2n=2x+1=17) together with an AFLP linkage map based on a cross between A. cepa and A. roylei Steam were used to re-evaluate the eight A. cepa linkage groups identified in the mapping study. The linkage groups could be assigned to individual, physical chromosomes. The low level of molecular homology between A. cepa and A. fistulosum enabled the identification of 186 amplified fragment length polymorphisms (AFLP(TM) markers) present in A. cepa and not in A. fistulosum with ten different primer combinations. With the monosomic addition lines the distribution of the markers over the eight chromosomes of A. cepa could be determined. Of these 186 AFLP markers 51 were absent in A. roylei and consequently used as markers in the mapping study (A. cepa x A. roylei cross). Therefore, these 51 AFLP markers could be used to assign the eight A. cepa linkage groups identified in the mapping study to physical chromosomes. Seven isozyme and three CAPS markers were also included. Two of the linkage groups had to be split because they included two sets of markers corresponding to different chromosomes. A total of 20 (approx. 10%) of the A. cepa-specific AFLP markers were amplified in more than one type of the monosomic addition lines, suggesting unlinked duplications. The co-dominant isozyme and CAPS markers were used to identify the correspondence of linkage groups originating from A. cepa or from A. roylei.