lines and bowl affect the specification of cyst stem cells and niche cells in the Drosophila testis
lines and bowl affect the specification of cyst stem cells and niche cells in the Drosophila testis
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DOI：
10.1242/dev.057364
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发表时间：
2011-05-01
期刊：
影响因子：
4.6
通讯作者：
Terry, Natalie
中科院分区：
文献类型：
--
作者：
DiNardo, Stephen;Okegbe, Tishina;Terry, Natalie
为了正常发挥功能，组织特异性干细胞必须驻留在一个小生境中。果蝇睾丸生态位是在体内研究的少数生态位之一。在这里，一个单一的小生境，包括十个枢纽细胞，保持生殖系干细胞（GSC）和体干细胞（CySC）。在这里，我们表明，线是一个重要的CySC因素。令人惊讶的是，细胞系耗尽的CySC采用了枢纽细胞的几个特征，包括新CySC的募集。这使我们研究了CySC和枢纽细胞之间的发育关系。与之前的报道相反，我们没有观察到稳态CySC子代向枢纽命运的显著转化。然而，我们发现，这两种细胞类型来自一个共同的前体池在性腺发育。此外，线突变胚胎表现出性腺含有多余的枢纽细胞，表明线抑制枢纽细胞的命运在性腺发生。在许多组织中，线的行为拮抗碗，我们发现，这是真实的枢纽规格，建立碗作为一个积极的作用因素，在睾丸生态位的发展。
To function properly, tissue-specific stem cells must reside in a niche. The Drosophila testis niche is one of few niches studied in vivo. Here, a single niche, comprising ten hub cells, maintains both germline stem cells (GSC) and somatic stem cells (CySC). Here, we show that lines is an essential CySC factor. Surprisingly, lines-depleted CySCs adopted several characteristics of hub cells, including the recruitment of new CySCs. This led us to examine the developmental relationship between CySCs and hub cells. In contrast to a previous report, we did not observe significant conversion of steady-state CySC progeny to hub fate. However, we found that these two cell types derive from a common precursor pool during gonadogenesis. Furthermore, lines mutant embryos exhibited gonads containing excess hub cells, indicating that lines represses hub cell fate during gonadogenesis. In many tissues, lines acts antagonistically to bowl, and we found that this is true for hub specification, establishing bowl as a positively acting factor in the development of the testis niche.