Comparisons of hyphal growth of Raffaelea quercivora among four Japanese Fagaceae species
Comparisons of hyphal growth of Raffaelea quercivora among four Japanese Fagaceae species
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四种日本壳斗科植物 Raffaelea quercivora 菌丝生长的比较
DOI：
10.1111/efp.12358
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发表时间：
2017
期刊：
影响因子：
1.4
通讯作者：
Ito S.
中科院分区：
文献类型：
--
作者：
Torii M.;Matsuda Y.;Ito S.
日本栎树枯萎病在日本引起广泛的栎树死亡。对致病真菌Raffaelea quercivora的易感性可能存在差异，这可能是由于寄主树的导管安排所致。为了弄清包括导管安排在内的本构防御机制或诱导防御机制是否是宿主易感性的主要决定因素，我们将真菌接种到四种日本蕨类植物的活苗或灭菌茎段中。在幼苗中，用染料评估水电导。用荧光显微镜观察幼苗和茎段菌丝的生长情况。在幼苗期，不同树种间茎横截面非导电边材面积和菌丝生长差异显著。在易感种栎（quercus crispulaaand serrata）中，菌丝生长与不导电边材比例呈显著正相关。在茎节中，菌丝的生长在种间或导管排列间无显著差异，与幼苗相似或大于幼苗。这些结果表明，边材的定殖程度由r。槲皮可作为易感性的标记物，其易感性主要由诱导的防御反应决定。
Japanese oak wilt causes widespread oak mortality in Japan. Possible differences in susceptibility to the causal fungus,Raffaelea quercivora, may be due to vessel arrangements in host trees. To clarify whether constitutive defence mechanisms including vessel arrangements or induced defence mechanisms are the main determinants of host susceptibility, we inoculated the fungus into living seedlings or sterilized stem segments of four Japanese fagaceous species. In seedlings, water conductance was assessed with dye. In both seedlings and stem segments, hyphal growth was examined by fluorescence microscopy. In seedlings, the area of non‐conductive sapwood in stem cross sections and hyphal growth differed significantly among species. In the susceptible speciesQuercus crispulaandQuercus serrata, hyphal growth was significantly and positively correlated with the proportion of non‐conductive sapwood. In stem segments, hyphal growth was not significantly different among species or between vessel arrangements and was similar to or greater than that in seedlings. These results suggest that the extent of sapwood colonization byR. quercivoracould be used as a marker for susceptibility and that susceptibility is determined mainly by induced defence responses.