Regulation of tumor invasion by HOXB13 gene overexpressed in human endometrial cancer.
Regulation of tumor invasion by HOXB13 gene overexpressed in human endometrial cancer.
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DOI：
10.3892/or.13.4.721
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发表时间：
2005-04
期刊：
影响因子：
4.2
通讯作者：
Yawei Zhao;T. Yamashita;M. Ishikawa
中科院分区：
文献类型：
--
作者：
Yawei Zhao;T. Yamashita;M. Ishikawa
在过去的二十年里，一组含有同源盒的基因，HOX基因家族，已经在胚胎发育和肿瘤的背景下进行了研究。特别是，越来越多的证据表明，包括乳腺癌在内的多种恶性肿瘤都与HOX异常有关。然而，关于HOX基因与子宫内膜癌的关系知之甚少，子宫内膜癌是女性生殖道最常见的恶性肿瘤，被认为与乳腺癌一样依赖于雌激素。在本研究中，我们通过实时荧光定量RT-PCR检测了HOXB13基因在子宫内膜癌细胞和患者组织中的过表达。为了研究HOXB13的过表达是否参与子宫内膜癌的侵袭或转移，我们采用电穿孔法将HOXB13/pcDNA3.1+反义质粒载体转染子宫内膜癌AN3CA细胞，并进行体外化学侵袭实验。我们发现反义转染的侵袭能力比亲本细胞和对照转染降低了90% （p<0.01）。此外，17 -雌二醇可诱导子宫内膜癌AN3CA细胞中HOXB13表达的时间和剂量依赖性反应。这些结果提示HOXB13在子宫内膜癌中的过表达可能与雌激素调控下癌细胞的侵袭能力有关。
During the last two decades, a group of homeobox-containing genes, the HOX gene family, has been studied both in the context of embryonic development and neoplasia. In particular, there is accumulating evidence of the involvement of HOX abnormalities in a variety of malignancies, including breast cancer. However, little is known about the association of HOX genes with endometrial cancer, which is the most common malignancy of the female genital tract and is thought to be dependent on estrogen, like breast cancer. In this study, we detected overexpression of the HOXB13 gene in endometrial cancer cells and tissues from patients by quantitative real-time RT-PCR. To investigate whether overexpression of HOXB13 is involved in invasion or metastasis of endometrial cancer, we transfected antisense HOXB13/pcDNA3.1+ plasmid vector into endometrial cancer AN3CA cells by electroporation and performed in vitro chemoinvasion assay. We revealed that the invasive ability of antisense-transfectants showed a 90% reduction compared with parental cells and control transfectants (p<0.01). In addition, administration of 17beta-estradiol induced time- and dose-dependent responses of the HOXB13 expression in endometrial cancer AN3CA cells. These results suggest that overexpression of HOXB13 in endometrial cancer may be associated with the invasive ability of cancer cells with regulation by estrogen.