INVOLVEMENT OF THYMOSIN-BETA-4 AND ENDOPROTEINASE ASP-N IN THE BIOSYNTHESIS OF THE TETRAPEPTIDE ACSERASPLYSPRO A REGULATOR OF THE HEMATOPOIETIC SYSTEM
INVOLVEMENT OF THYMOSIN-BETA-4 AND ENDOPROTEINASE ASP-N IN THE BIOSYNTHESIS OF THE TETRAPEPTIDE ACSERASPLYSPRO A REGULATOR OF THE HEMATOPOIETIC SYSTEM
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DOI：
10.1016/0014-5793(90)81322-f
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发表时间：
1990-11-12
期刊：
影响因子：
3.5
通讯作者：
LENFANT, M
中科院分区：
文献类型：
--
作者：
GRILLON, C;RIEGER, K;LENFANT, M
结果表明，胸腺肽β4在体内外可通过一步酶切产生新的造血系统调节因子AcSDKP。在骨髓细胞中均检测到AcSDKP和T-β-4。[~3H]T-β-4与完整或裂解的骨髓基质细胞孵育均可形成[~3H]AcSDKP，而标记的四肽在此条件下不被降解。用细菌酶对Tβ4进行的模拟酶降解表明，哺乳动物内切酶Asp-N可能通过对Tβ4的4Pro-5Asp多肽键的特异性裂解参与了AcSDKP的形成。
It is shown that AcSDKP a new regulator of the hematopoietic system can he generated from thymosin β4by a one-step enzymatic cleavage in vitro and in vivo. AcSDKP and Tβ4were both detected in bone marrow cells (BMC'). Incubation of [3H]Tβ4with either intact or lysed BMC led to the formation of [3H]AcSDKP whereas the labelled tetrapeptide was not degraded under these conditions. Model enzymatic degradation of Tβ4carried out with bacterial enzymes suggests that a mammalian endoproteinase Asp-N might be involved in the formation of AcSDKP through the specific cleavage of the4Pro-5Asp peptide bond of T β4.