EXPRESSION OF THE DROSOPHILA GOOSEBERRY LOCUS DEFINES A SUBSET OF NEUROBLAST LINEAGES IN THE CENTRAL-NERVOUS-SYSTEM
EXPRESSION OF THE DROSOPHILA GOOSEBERRY LOCUS DEFINES A SUBSET OF NEUROBLAST LINEAGES IN THE CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1006/dbio.1995.1219
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发表时间：
1995-08-01
影响因子：
2.7
通讯作者：
HOLMGREN, R
中科院分区：
文献类型：
--
作者：
BUENZOW, DE;HOLMGREN, R
众所周知，中枢神经系统的发育需要在神经母细胞及其后代中表达的谱系特异性因子，以及参与细胞-细胞信号机制的分子。由醋栗基因编码的转录因子似乎是适当规范神经母细胞及其谱系所必需的。为了检验醋栗的表达是否具有谱系特异性，我们利用酵母的FLP重组酶对果蝇整个发育过程中的细胞谱系进行了积极的标记。在这个系统中，actin5C启动子和lacZ基因被一个由两个直接重复的FRT组成的多聚腺苷酸化信号隔开。热休克被用来诱导FLP重组酶的脉冲，该脉冲催化FRT位点之间的位点特异性重组事件。由此产生的聚腺苷酸化位点的切除允许从actin5C启动子表达LacZ。经历了重组事件的细胞的后代现在被正面标记，使我们能够将细胞谱系与醋栗基因表达模式进行比较。我们发现，猕猴桃基因的表达具有谱系特异性，提示猕猴桃基因可能在果蝇中枢神经系统的模式中起到选择基因的作用。(C)1995年学术出版社。
The development of the central nervous system is known to require lineage-specific factors that are expressed in neuroblasts and their descendants, as well as molecules involved in cell-cell signaling mechanisms. The transcription factors encoded by the gooseberry locus appear to be required for the proper specification of neuroblasts and their lineages. To examine whether gooseberry expression is lineage-specific, we have utilized the FLP recombinase of yeast to positively mark cell lineages throughout Drosophila development. In this system, the actin5C promoter and the lacZ gene are separated by a polyadenylation signal flanked by two direct repeat FRTs. A heat shock is used to induce a pulse of FLP recombinase which catalyzes a site-specific recombination event between the FRT sites. The resulting excision of the polyadenylation site allows expression of lacZ from the actin5C promoter. The descendants of a cell which has undergone a recombination event are now positively marked, enabling us to compare cell lineages with the pattern of gooseberry gene expression. We find that the expression of the gooseberry locus is lineage-specific, suggesting that gooseberry may function as a selector gene in the patterning of the Drosophila central nervous system. (C) 1995 academic press, Inc.