Synovial nitric oxide concentrations are increased and correlated with serum levels in patients with active Behcet's disease:: a pilot study
Synovial nitric oxide concentrations are increased and correlated with serum levels in patients with active Behcet's disease:: a pilot study
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DOI：
10.1007/s10067-004-1015-3
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发表时间：
2005-08-01
影响因子：
3.4
通讯作者：
Ascioglu, O
中科院分区：
文献类型：
--
作者：
Duygulu, F;Evereklioglu, C;Ascioglu, O
白塞病（BD）是一种复发性免疫炎症性血管炎，病因不明，以内皮功能障碍为特征。约75%的病例出现关节症状和体征，以血清阴性关节炎和非特异性滑膜炎为特征。我们证实，血清和红细胞一氧化氮（NO中心点）水平（体内最丰富的自由基）在BD中升高，并与疾病活动相关。本研究进一步调查了活动性和非活动性双相障碍患者滑液和血清中的NO中心点水平。共有23名符合国际研究组标准的关节受累的双相障碍患者（14男9女）和15名年龄和性别匹配的健康对照组（9男6女）接受了选择性关节镜检查。滑液和血清分别取自BD患者和对照组。临床和实验室结果包括中性粒细胞计数和红细胞沉降率（ESR）将BD患者分为活动性（n = 11）和非活动性（n = 12）。比较两组间滑膜及血清NO中心点水平，并进行相关性分析。BD患者急性期反应物水平显著高于对照组（p< 0.01）。活动期白塞病患者滑膜NO中心点水平（平均+/- SD 76.61 +/- 11.95 mu mol/l）显著高于活动期白塞病患者（46.16 +/- 8.89 mu mol/l, p< 0.001）和健康对照组（39.60 +/- 8.03 mu mol/l, p< 0.001）。不运动组与对照组比较差异无统计学意义（p < 0.05）。活动期BD患者血清NO中心点水平（38.84 +/- 9.15 mu mol/l）显著高于活动期患者（30.91 +/- 5.88 mu mol/l, p= 0.018）和对照组（28.86 +/- 5.91 mu mol/l, p=0.002）。滑膜NO中心点水平与血清NO水平呈正相关（r(2) = 0.621, p< 0.001）。活动期双相障碍患者滑膜NO中心点水平升高可能反映了滑膜的非特异性炎症过程，因此关节痛和关节炎是双相障碍的常见表现。
Behcet's disease (BD) is a relapsing immuno-inflammatory vasculitis of unknown etiology characterized by endothelial dysfunction. Articular symptoms and signs are present in about 75% of cases and characterized by seronegative arthritis and nonspecific synovitis. We demonstrated that both serum and erythrocyte nitric oxide (NO center dot) levels, the most abundant free radical in the body, were elevated in BD and associated with disease activity. This study further investigated NO center dot levels in the synovial fluid and serum from patients with active and inactive BD. A total of 23 BD patients with articular involvement ( 14 men and 9 women) satisfying International Study Group criteria and 15 age- and sex-matched healthy control subjects ( 9 men and 6 women) undergoing elective arthroscopy were included in this case-control investigation. The synovial fluid and serum were obtained from BD patients and controls. Clinical and laboratory findings including neutrophil count and erythrocyte sedimentation rate (ESR) were used to classify BD patients as active (n = 11) or inactive (n = 12). Synovial as well as serum NO center dot levels were compared between the groups and correlation analysis was performed. Acute phase reactant levels were significantly higher ( for each, p< 0.01) in BD patients than control subjects in the active period. The mean synovial NO center dot level in active Behcet's patients ( mean +/- SD 76.61 +/- 11.95 mu mol/l) was significantly higher than in inactive patients (46.16 +/- 8.89 mu mol/l, p< 0.001) and healthy control subjects (39.60 +/- 8.03 mu mol/l, p< 0.001). The difference between inactive patients and controls was not significant ( p> 0.05). Active BD patients had significantly higher serum NO center dot levels ( 38.84 +/- 9.15 mu mol/l) than inactive patients (30.91 +/- 5.88 mu mol/ l, p = 0.018) and control subjects (28.86 +/- 5.91 mu mol/ l, p=0.002). In addition, synovial NO center dot levels were positively correlated with serum levels (r(2) = 0.621, p< 0.001). Increased synovial NO center dot levels in active BD patients probably reflect a nonspecific inflammatory process of the synovium and, therefore, arthralgia and arthritis as a common finding of BD.