Role of a novel pathogen-induced pepper C3-H-C4 type RING-finger protein gene, CaRFP1, in disease susceptibility and osmotic stress tolerance
Role of a novel pathogen-induced pepper C3-H-C4 type RING-finger protein gene, CaRFP1, in disease susceptibility and osmotic stress tolerance
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DOI：
10.1007/s11103-006-9110-2
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发表时间：
2007-03-01
影响因子：
5.1
通讯作者：
Hwang, Byung Kook
中科院分区：
文献类型：
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作者：
Hong, Jeum Kyu;Choi, Hyong Woo;Hwang, Byung Kook
关于环指蛋白在植物防御中的作用的信息有限。从受野油菜黄单胞菌致病变种（Xanthomonascampestrispv.）侵染的辣椒叶片中分离到一个编码C3-H-C4型环指蛋白（RING-finger protein，RING-finger protein，RING-finger protein，RING-finger protein）的辣椒CaRFP 1。囊膜CaRFP 1蛋白具有VWFA结构域、N端富含丝氨酸的区域和C端富含半胱氨酸的区域。在辣椒叶片与X.野油菜致病变种vesicatoria，以及在系统的，未接种的辣椒叶组织。CaRFP 1基因在辣椒炭疽病菌侵染的叶片组织中也被诱导表达。CaRFP 1基因强烈诱导水杨酸，乙烯和茉莉酸甲酯处理，以及环境胁迫，包括甲基紫精，甘露醇和NaCl处理早得多。CaRFP 1基因在转基因拟南芥植株中的过表达赋予了对假单胞菌pv.番茄感染，伴随PR-2和PR-5基因表达的减少，表明CaRFP 1作为E3连接酶用于靶PR蛋白的聚泛素化。外源水杨酸处理也抑制了PR-2和PR-5基因在转基因植株中的表达。在萌发和幼苗发育过程中，CaRFP 1过表达植株对高盐和干旱的耐受性不同，这与拟南芥对脱落酸的敏感性密切相关。这些结果表明，CaRFP 1基因的功能作为一个早期的防御调节控制细菌疾病的易感性和渗透胁迫的耐受性。
Limited information is available about the roles of RING-finger proteins in plant defense. A pepper CaRFP1 encoding the C3-H-C4 type RING-finger protein that physically interacted with the basic PR-1 protein CABPR1 was isolated from pepper leaves infected by Xanthomonas campestris pv. vesicatoria. The CaRFP1 protein has VWFA domain, and N-terminal serine-rich and C-terminal cysteine-rich regions. The CaRFP1 transcripts accumulated earlier than did those of the basic PR-1 gene CABPR1 during the incompatible interaction of pepper leaves with X. campestris pv. vesicatoria, as well as in the systemic, uninoculated pepper leaf tissues. The CaRFP1 gene also was induced in pepper leaf tissues infected by Colletotrichum coccodes. The CaRFP1 gene was strongly induced much earlier by salicylic acid, ethylene and methyl jasmonate treatments, as well as environmental stresses including methyl viologen, mannitol and NaCl treatments. Overexpression of the CaRFP1 gene in the transgenic Arabidopsis plants conferred disease susceptibility to Pseudomonas syringae pv. tomato infection, accompanied by reduced PR-2 and PR-5 gene expression, suggesting that the CaRFP1 acts as an E3 ligase for polyubiquitination of target PR proteins. Exogenous salicylic acid treatment also abolished PR-2 and PR-5 gene expression in the transgenic plants. Differential osmotic stress tolerance was induced by high salt and drought in the CaRFP1-overexpressing plants during germination and seedling development, which was closely correlated with abscisic acid sensitivity of Arabidopsis plants. These results suggest that the CaRFP1 gene functions as an early defense regulator controlling bacterial disease susceptibility and osmotic stress tolerance.