In-vitro penetration of pig oocytes matured in culture by frozenp=n- hawed ejaculated spermatozoa
In-vitro penetration of pig oocytes matured in culture by frozenp=n- hawed ejaculated spermatozoa
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冷冻p=n-解冻的射精精子对培养成熟的猪卵母细胞的体外渗透
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发表时间：
2008
期刊：
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通讯作者：
K. Okuda
中科院分区：
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作者：
W. H. Wang;K. Niwa;K. Okuda
摘要将培养中成熟的猪卵母细胞用冷冻的p =n- 在改良的组织培养基（TCM）中未预孵育的情况下取出射出的精子199。在25 p =n-100 m=x 106精子/ml时获得了高穿透率（85 p =n-89%）和多精受精发生率增加。在含5 mm咖啡因的培养基中授精的卵母细胞中观察到穿透率的广泛变化（16 p =n-89%），并在25 p =n-50 m=x 106精子/ml从6头公猪中获得，无论精子活力如何。在25 p =n-50 m=x 106精子/ml时，卵母细胞的穿透率取决于培养基中咖啡因的浓度：无咖啡因时没有穿透，但5 mm咖啡因的穿透率最高（89%）。没有卵母细胞在补充有5 p =n-40 g=mg/ml肝素的培养基中穿透。当肝素与5 mm咖啡因一起加入时，它抑制了咖啡因促进精子穿透卵母细胞的功效。
Summary. Pig oocytes matured in culture were inseminated with frozenp=n- hawed ejaculated spermatozoa without preincubation in modified tissue culture medium (TCM) 199. High penetration rates (85p=n-89%) and increased incidence of polyspermy were obtained at 25p=n-100 m=x 106 spermatozoa/ml. Wide variation in penetration rates (16p=n-89%) was observed in oocytes inseminated in medium containing 5mm caffeine and at 25p=n-50 m=x 106 spermatozoa/ml obtained from 6 boars, regardless of sperm motility. At 25p=n-50 m=x 106 spermatozoa/ml, penetration rates of oocytes were dependent upon the concentration of caffeine in the medium: there was no penetration without caffeine, but penetration was highest (89%) with 5mm caffeine. None of the oocytes was penetrated in the medium supplemented with heparin at 5p=n-40 g=mg/ml. When heparin was included in the medium with 5mm caffeine, it inhibited the efficacy of caffeine to promote sperm penetration of oocytes.