Angiogenic activity of osteopontin-derived peptide SVVYGLR
Angiogenic activity of osteopontin-derived peptide SVVYGLR
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DOI：
10.1016/j.bbrc.2003.09.001
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发表时间：
2003-10-10
影响因子：
3.1
通讯作者：
Matsuura, N
中科院分区：
文献类型：
--
作者：
Hamada, Y;Nokihara, K;Matsuura, N
血管生成在各种病理条件以及一些生理过程中起着重要作用。尽管已有大量可溶性血管生成因子的报道，但细胞外基质也通过与内皮细胞的相互作用对血管生成起着至关重要的作用。由于最近的研究表明骨桥蛋白具有一定的血管生成活性，本研究探讨了骨桥蛋白分子中新的结合基序SVVYGLR肽对血管生成的影响。合成肽SVVYGLR对内皮细胞无增殖作用，但具有粘附和迁移活性。此外，SVVYGLR在三维胶原凝胶中具有与血管内皮生长因子（已知是最强的血管生成因子）同样有效的管形成活性。电镜观察显示，SVVYGLR可诱导内皮细胞孔化和分化，内皮细胞表面可见大量微绒毛，内皮细胞间有紧密连接形成。这种小分子肽由于分子量小而具有一定的优势，有望在将来刺激血管生成以改善某些缺血性疾病。(C)2003年爱思唯尔公司All rights reserved.
Angiogenesis plays an important role in various pathological conditions as well as some physiological processes. Although a number of soluble angiogenic factors have been reported, extracellular matrix also has crucial effect on angiogenesis through interaction with endothelial cells. Since recent reports showed osteopontin had some angiogenic activity, the effect of the SVVYGLR peptide, novel binding motif in osteopontin molecule, on angiogenesis was examined in this study. Synthetic peptide SVVYGLR did not have proliferative effect on endothelial cells but adhesion and migration activity to endothelial cells. Furthermore, SVVYGLR had as potent activity for tube formation in three-dimensional collagen gel as vascular endothelial growth factor which is known to be the strongest angiogenic factor. Electron microscopical analysis showed a number of microvilli on the endothelial luminar surface and tight junction formation in the luminar intercellular border between endothelial cells, indicating SVVYGLR induced cell porarity and differentiation of endothelial cells. This small peptide might be expected to stimulate angiogenesis to improve some ischemic conditions in the future because of some advantages due to smaller molecular weight. (C) 2003 Elsevier Inc. All rights reserved.