Amphiphilic cholic-acid-modified dextran sulfate and its application for the controlled delivery of superoxide dismutase.
Amphiphilic cholic-acid-modified dextran sulfate and its application for the controlled delivery of superoxide dismutase.
复制标题
DOI：
10.1002/mabi.201100367
复制
发表时间：
2012-04
影响因子：
4.6
通讯作者：
Y. Xiong;Jianing Qi;Ping Yao
中科院分区：
文献类型：
--
作者：
Y. Xiong;Jianing Qi;Ping Yao
以DS和CA为原料，通过酯化反应合成了一种新型的两亲性可生物降解的双马来酰亚胺DS-CA。DS-CA可以在水中自组装成稳定的纳米颗粒。在pH = 5.0时，SOD与DS-CA通过静电相互作用和疏水相互作用能有效结合。在pH = 1.2时，复合纳米颗粒的SOD释放缓慢。在pH = 7.4的PBS中的释放表现出延长的行为，并且通过改变SOD与DS-CA的重量比以及CA取代度来调节。提高DS-CA的CA取代度可显著提高细胞对负载SOD的摄取。该研究表明，两亲性DS-CA为蛋白质/肽类药物的口服递送提供了一种有前途的策略。
A novel amphiphilic and biodegradable polyelectrolyte DS-CA is prepared by the esterification of DS with CA. DS-CA can self-assemble into stable nanoparticles in water. SOD can effectively associate with DS-CA at pH = 5.0 by virtue of electrostatic and hydrophobic interactions. SOD release from the complex nanoparticles is slow at pH = 1.2. The release at pH = 7.4 PBS shows an extended behavior and is tunable by changing the weight ratio of SOD to DS-CA as well as the CA substitution degree. Increasing the CA substitution degree of DS-CA can significantly enhance the cellular uptake of the loaded SOD. This study demonstrates that the amphiphilic DS-CA provides a promising strategy for oral delivery of protein/peptide drugs.