The role of the carboxyterminal domain of RNA polymerase II in regulating origins of DNA replication in Saccharomyces cerevisiae.
The role of the carboxyterminal domain of RNA polymerase II in regulating origins of DNA replication in Saccharomyces cerevisiae.
复制标题
RNA 聚合酶 II 的羧基末端结构域在调节酿酒酵母 DNA 复制起点中的作用。
DOI：
10.1093/genetics/162.3.1117
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发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Yankulov,Krassimir
中科院分区：
文献类型：
--
作者：
Gauthier,Laura;Dziak,Renata;Kramer,DavidJH;Leishman,David;Song,Xiaomin;Ho,Jason;Radovic,Maja;Bentley,David;Yankulov,Krassimir
MCM（minichromosome maintenance）蛋白作为复制许可因子（replication licensing factor，RLF-M）发挥作用，其有助于将DNA复制的起始限制为每个细胞周期一次。在本研究中，我们表明，截断的pol II CTD在aS。一株在mcm 5中含有突变的大肠杆菌部分逆转了其表型，并改善了CEN/ARS微型染色体的维持。我们将这种表型与对DNA复制的影响相关联，而不是与对转录或特定基因表达的影响相关联。我们还表明，类似的截断CTD降低微型染色体的稳定性和损害刺激DNA复制的反式激活剂和拴系的重组聚合物II CTD复制的起点有显着的刺激作用的微型染色体的稳定性。此外，我们表明，polII被招募到ARS 1。我们认为，inS. polaea的pol II转录和DNA复制的协调机制是由pol II的CTD介导的。
MCM (minichromosome maintenance) proteins function as a replication licensing factor (RLF-M), which contributes to limiting initiation of DNA replication to once per cell cycle. In the present study we show that a truncation of the pol II CTD in aS. cerevisiaestrain harboring a mutation inmcm5partially reverses itstsphenotype and improves maintenance of CEN/ARS minichromosomes. We correlate this phenotype to effects on DNA replication rather than to effects on transcription or specific gene expression. We also demonstrate that a similar truncation of the CTD reduces minichromosome stability and impairs stimulation of DNA replication bytrans-activators and that tethering of recombinant pol II CTD to an origin of replication has a significant stimulatory effect on minichromosome stability. Furthermore, we show that pol II is recruited to ARS1. We propose that inS. cerevisiaea mechanism of coordinating pol II transcription and DNA replication is mediated by the CTD of pol II.