How does ultrasonic cavitation remove dental bacterial biofilm?
How does ultrasonic cavitation remove dental bacterial biofilm?
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DOI：
10.1016/j.ultsonch.2020.105112
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发表时间：
2020-10-01
影响因子：
8.4
通讯作者：
Walmsley, A. D.
中科院分区：
文献类型：
--
作者：
Vyas, N.;Wang, Q. X.;Walmsley, A. D.
细菌生物膜积累在许多领域都是有问题的，导致海洋环境和食品工业中的生物污染以及医疗保健中的感染。生物膜的物理破坏已成为一个重要的研究领域。在牙科中，生物膜的去除对保持健康至关重要。本研究的目的是使用高速摄像机观察由牙科超声洁牙机（P5XS，Acteon）产生的空化引起的生物膜破坏，并确定如何实现这一点。血链球菌生物膜在ThermanoxTM盖玻片（Nunc，USA）上生长4天。固定并用结晶紫染色后，使用高速照相机（AX200，Photron）对生物膜去除进行成像。将超声洁牙器尖端（尖端10P）保持在距离生物膜2mm处并操作2秒。从高速图像序列中观察到气泡振荡，并使用图像分析来跟踪气泡运动并计算气泡半径和表面速度的变化。结果表明，无论是单独还是在空化云中，大多数生物膜破坏都是通过空化气泡在2秒内接触表面而发生的。通过表面上的形状振荡微泡以及通过流体流动进行清洁。
Bacterial biofilm accumulation is problematic in many areas, leading to biofouling in the marine environment and the food industry, and infections in healthcare. Physical disruption of biofilms has become an important area of research. In dentistry, biofilm removal is essential to maintain health. The aim of this study is to observe biofilm disruption due to cavitation generated by a dental ultrasonic scaler (P5XS, Acteon) using a high speed camera and determine how this is achieved. Streptococcus sanguinis biofilm was grown on Thermanox (TM) coverslips (Nunc, USA) for 4 days. After fixing and staining with crystal violet, biofilm removal was imaged using a high speed camera (AX200, Photron). An ultrasonic scaler tip (tip 10P) was held 2 mm away from the biofilm and operated for 2 s. Bubble oscillations were observed from high speed image sequences and image analysis was used to track bubble motion and calculate changes in bubble radius and velocity on the surface. The results demonstrate that most of the biofilm disruption occurs through cavitation bubbles contacting the surface within 2 s, whether individually or in cavitation clouds. Cleaning occurs through shape oscillating microbubbles on the surface as well as through fluid flow.