Biomimetic niche for neural stem cell differentiation using poly-L-lysine/hyaluronic acid multilayer films
Biomimetic niche for neural stem cell differentiation using poly-L-lysine/hyaluronic acid multilayer films
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DOI：
10.1177/0885328214563341
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发表时间：
2015-05-01
影响因子：
2.9
通讯作者：
Yang, Wen-Ting
中科院分区：
文献类型：
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作者：
Lee, I-Chi;Wu, Yu-Chieh;Yang, Wen-Ting
聚电解质多层膜已被建议作为具有柔性表面性质的可调基底，其可以调节电池行为。然而，这些膜对神经干细胞/祖细胞的生物学效应很少被研究。在此，选择由透明质酸和聚-L-赖氨酸组成的仿生多层膜来模拟脑组织的天然细胞外基质生态位，并评估其诱导效果，而不添加化学因子。由于神经干/祖细胞对基质性质敏感，因此该系统提供对表面电荷的控制是重要的，并且轻微的刚度变化也是可能的。这两个因素都影响神经干/祖细胞分化。结果表明，神经干/祖细胞在0.5-4层交替的聚L-赖氨酸/透明质酸多层膜上可诱导分化。此外，轴突生长长度受终末层表面电荷的调节，但不随层数的增加而增加。与此相反，分化的神经元的数量略有增加，随着层数的增加，但不受终末层的表面电荷。总之，天然聚-L-赖氨酸/透明质酸膜形式的材料对实现了神经再生医学的重要目标，包括神经突生长长度的增强、神经元分化的调节和网络的形成。这些细胞外基质模拟聚-L-赖氨酸/透明质酸多层膜可以提供一个通用的平台，可以用于神经工程中的应用程序的表面修饰。
Polyelectrolyte multilayer films have been suggested as tunable substrates with flexible surface properties that can modulate cell behavior. However, these films' biological effects on neural stem/progenitor cells have rarely been studied. Herein, biomimetic multilayer films composed of hyaluronic acid and poly-L-lysine were chosen to mimic the native extracellular matrix niche of brain tissue and were evaluated for their inductive effects, without the addition of chemical factors. Because neural stem/progenitor cells are sensitive to substrate properties, it is important that this system provides control over the surface charge, and slight stiffness variations are also possible. Both of these factors affect neural stem/progenitor cell differentiation. The results showed that neural stem/progenitor cells were induced to differentiate on the poly-L-lysine/hyaluronic acid multilayer films with 0.5-4 alternating layers. In addition, the neurite outgrowth length was regulated by the surface charge of the terminal layer but did not increase with the layer number. In contrast, the quantity of differentiated neurons was enhanced slightly as the number of layers increased but was not affected by the surface charge of the terminal layer. In sum, material pairs in the form of native poly-L-lysine/hyaluronic acid films achieved important targets for neural regenerative medicine, including enhancement of the neurite outgrowth length, regulation of neuron differentiation, and the formation of a network. These extracellular matrix-mimetic poly-L-lysine/hyaluronic acid multilayer films may provide a versatile platform that could be useful for surface modification for applications in neural engineering.