MdWRKY30, a group IIa WRKY gene from apple, confers tolerance to salinity and osmotic stresses in transgenic apple callus and Arabidopsis seedlings
MdWRKY30, a group IIa WRKY gene from apple, confers tolerance to salinity and osmotic stresses in transgenic apple callus and Arabidopsis seedlings
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MdWRKY30 是来自苹果的 IIa 组 WRKY 基因，赋予转基因苹果愈伤组织和拟南芥幼苗对盐度和渗透胁迫的耐受性
DOI：
10.1016/j.plantsci.2020.110611
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发表时间：
2020-10-01
期刊：
影响因子：
5.2
通讯作者：
Ma, Fengwang
中科院分区：
文献类型：
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作者：
Dong, Qinglong;Zheng, Wenqian;Ma, Fengwang
非生物胁迫威胁着经济上重要的多年生水果作物如苹果（Malus x domestica Borkh.）的生产力和质量。WRKY转录因子在植物对非生物胁迫的响应中发挥着多种作用，但对苹果WRKY基因的研究知之甚少。在这里，我们进行了苹果IIa组WRKY基因（MdWRKY30）的功能表征。qRT-PCR分析发现，盐胁迫和干旱胁迫诱导了MdWRKY30的表达。亚细胞定位实验表明，MdWRKY30定位于细胞核。反激活实验发现，MdWRKY30没有转录激活活性。Y2H分析表明，MdWRKY26、MdWRKY28和MdWRKY30相互作用形成异源二聚体和同源二聚体。转基因分析表明，MdWRKY30在拟南芥中的过表达增强了幼苗期、种子萌发期和子叶变绿期的耐盐性和渗透性。MdWRKY30过表达通过转录调控胁迫相关基因增强转基因苹果愈伤组织对盐胁迫和渗透胁迫的耐受性。综上所述，我们的研究结果表明，MdWRKY30是苹果耐盐性和渗透胁迫性的重要调节因子。
Abiotic stresses threaten the productivity and quality of economically important perennial fruit crops such as apple (Malus x domestica Borkh.). WRKY transcription factors play various roles in plant responses to abiotic stress, but little is known regarding WRKY genes in apple. Here, we carried out functional characterization of an apple Group IIa WRKY gene (MdWRKY30). qRT-PCR analysis found that MdWRKY30 expression was induced by salt and drought stress. A subcellular localization assay showed that MdWRKY30 is localized to the nucleus. A transactivation assay found that MdWRKY30 has no transcriptional activation activity. A Y2H assay indicated that MdWRKY26, MdWRKY28, and MdWRKY30 interact with each other to form heterodimers and homodimers. Transgenic analysis revealed that the overexpression of MdWRKY30 in Arabidopsis enhanced salt and osmotic tolerance in the seedling stage, as well as during the seed germination and greening cotyledon stages. MdWRKY30 overexpression enhanced tolerance to salt and osmotic stresses in transgenic apple callus through transcriptional regulation of stress-related genes. Together, our results demonstrate that MdWRKY30 is an important regulator of salinity and osmotic stress tolerance in apple.