Nitric oxide limits transcriptional induction of nitric oxide synthase in CNS glial cells.
Nitric oxide limits transcriptional induction of nitric oxide synthase in CNS glial cells.
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DOI：
10.1006/bbrc.1994.1766
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发表时间：
1994-06
影响因子：
3.1
通讯作者：
Song Kyu Park;Hain Lee Lin;S. Murphy
中科院分区：
文献类型：
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作者：
Song Kyu Park;Hain Lee Lin;S. Murphy
一氧化氮(NO)可直接或间接调节多种酶的活性，其中包括一氧化氮合酶(NOS)。此外，星形胶质细胞在细胞因子的转录诱导下形成的NO似乎影响着NOS的mRNA水平。我们已经观察到，在存在一氧化氮合酶抑制剂或一氧化氮捕捉剂的诱导下，一氧化氮合酶的表达增强，并且这种作用被一氧化氮供体逆转。NO似乎抑制了一氧化氮合酶的转录诱导，而不是促进mRNA的不稳定性。NO对一氧化氮合酶基因诱导的这种作用提供了一种机制，通过该机制可以精细地调节NO在神经系统中的时间和空间产生，NO是一种反应性的和潜在的毒性介质。
Nitric oxide (NO) can modulate directly and indirectly the activity of a variety of enzymes, including nitric oxide synthase (NOS). In addition, it appears that the NO formed by astroglial cells in which transcriptional induction of NOS has been evoked by cytokines affects NOS mRNA level. We have observed that induction in the presence of NOS inhibitors or NO-trapping agents amplifies NOS mRNA expression and that this effect is reversed by an NO donor. Rather than promoting mRNA instability, NO appears to inhibit the transcriptional induction of NOS. This effect of NO on induction of the NOS gene provides a mechanism by which the temporal and perhaps spatial production in the nervous system of NO, a reactive and potentially toxic mediator, can be finely regulated.