ACUTE GONADOTROPIN DEPRIVATION .1. A MODEL FOR THE STUDY OF FOLLICULAR ATRESIA
ACUTE GONADOTROPIN DEPRIVATION .1. A MODEL FOR THE STUDY OF FOLLICULAR ATRESIA
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DOI：
10.1095/biolreprod24.4.913
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发表时间：
1981-01-01
影响因子：
3.6
通讯作者：
GREENWALD, GS
中科院分区：
文献类型：
--
作者：
BILL, CH;GREENWALD, GS
本文介绍了一种用于研究有腔卵泡闭锁的模型。用孕马血清(PMS)和兔抗PMS抗体(PMS-AS)诱导去垂体仓鼠卵泡发育和闭锁。成年仓鼠在第一天(发情期)切除脑垂体；手术后每只动物接受30IU的PMS(Sc)。第4天腹腔注射PMS-AS(100 IL，未稀释)，第2~3天血清E_2升高最大，第4天0900h达到最大值1030±164(均值±扫描电子显微镜)pg/ml，第5天E_1浓度(未加PMS-AS)无明显变化，P浓度从第3天的6.1±1.5 ng/ml降至第4天的1.7±0.3 ng/ml，提示黄体功能减退。根据血清PMS的放射免疫分析，卵泡对促性腺激素的支持在第4天达到最大。在第4-5天，经前综合征的血清浓度下降了50%。PMS-AS治疗1h后，血清雌二醇降至461±80pg/ml。2 h时浓度为150±6 pg/mi，8 h时为45±2 pg/mi，24 h时已检测不到激素。血清孕酮在第4天下降，在PMS-AS后24小时内无明显变化。PMS-AS处理后4h即可见颗粒细胞核固缩。颗粒细胞的变性始于卵丘，然后扩散到周围细胞。24 h时，卵母细胞核膜消失，在减数分裂的不同阶段可见染色质，但未见极体。
A model for the study of atresia in antral follicles is described. Follicular development and atresia was induced in hypophysectomized hamsters using pregnant mares serum (PMS) followed by rabbit-anti-PMS(PMS-As). Adult hamsters were hypophysectomized on Day 1 (metestrus); following surgery each animal received 30 IU of PMS(sc). PMS-As (100 il, undiluted) was administered ip on Day 4.Serum E2 underwent the greatest increase between Days 2 and 3, reaching a maximal concentration of 1030±164 (mean±SEM) pg/mi at 0900 h on Day 4. There was no change in E, concentration(in the absence of PMS-As) on Day 5. Serum P concentration fell from 6.1±1.5 ng/ml on Day 3 to 1.7±0.3 ng/ml on Day 4, indicating luteal regression. Gonadotropin support of follicles was maximal through Day 4, as determined by radioimmunoassay of serum PMS. Between Days 4 and 5 the serum concentration of PMS dropped by 50%. One hour following treatment with PMS-As, serum estradiol fell to 461±80 pg/mI. By 2 h the concentration was 150±6 pg/mI; by 8 h, 45±2 pg/mI; and by 24 h the hormonewas undetectable. Serum progesterone had dropped by Day 4 and did not change significantly over the 24 h interval following PMS-As. Pyknosis of granulosa cell nuclei was first observed 4 h following PMS-As treatment. Granulosa cell degeneration began in the cumulus oophorus and then spread to the peripheral cells. By 24 h the nuclear envelope of the oocyte disappeared and the chromatin was seen in various stages of meiotic division; however, no polar bodies were observed.