An estrogen binding protein in the renal cytosol of intact, castrated and estrogenized golden hamsters.
An estrogen binding protein in the renal cytosol of intact, castrated and estrogenized golden hamsters.
复制标题
完整、去势和雌激素化金仓鼠肾细胞质中的雌激素结合蛋白。
作者：
J. Li;D. Talley;S. Li;C. Villee
通过蔗糖密度梯度分析，与卵清蛋白和牛血清白蛋白相比，未处理的正常、去势和雌激素处理的仓鼠肾细胞溶胶中存在的雌激素结合蛋白具有4S的沉降值。随着雌激素治疗时间的延长，结合量增加。结合位点表现出对雌激素的特异性（例如，己烯雌酚、雌酮、雌三醇、雌二醇-17 α），因为在存在这些未标记雌激素的情况下，3 H-雌二醇结合显著降低，但在存在双氢睾酮、醛固酮、皮质醇、孕酮和孕酮代谢物的情况下，3 H-雌二醇结合不显著降低。这种雌激素结合大分子的性质通过其与硫酸鱼精蛋白的沉淀进一步确定。尽管肾脏中雌激素的这种特异性大分子结合比仓鼠子宫中的小得多，但它比血浆、肺或肝中的大得多。去势大鼠肾和肝细胞液中特异性雌二醇结合量。
An estrogen binding protein present in the renal cytosols of untreated normal, castrated and estrogen-treated hamsters has a sedimentation value of 4S by sucrose density gradient analysis when compared to ovalbumin and bovine serum albumin. The amount of binding was enhanced with prolonged estrogen treatment. The binding site exhibits specificity for estrogenic hormone (e.g., diethylstilbesterol, estrone, estriol, estradiol-17α) since 3H-estradiol binding is decreased markedly in the presence of these unlabeled estrogens but not with dihydrotestosterone, aldosterone, cortisol, progesterone and metabolites of progesterone. The nature of this estrogen binding macromolecule was further ascertained by its precipitation with protaminesulfate. Although this specific macromolecular binding by estrogens in the kidney is much less than in the hamster uterus, it is considerably greater than that in the plasma, lung or liver. The amount of specific estradiol binding in the kidney and liver cytosols of castrated rats...