A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants
A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants
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DOI：
10.1073/pnas.102301599
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发表时间：
2002-08-06
影响因子：
11.1
通讯作者：
Hanson, MR
中科院分区：
文献类型：
--
作者：
Bentolila, S;Alfonso, AA;Hanson, MR
在150多个物种中，细胞质雄性不育是由异常的线粒体基因编码的，这种基因会阻止花粉的发育。大多数编码细胞质雄性不育的线粒体基因的RNA或蛋白质水平表达在一个或多个被称为生育恢复者的核基因的存在下被改变，这些核基因抑制雄性不育表型。细胞质雄性不育/恢复系统已被证明是杂交种子生产的宝贵工具。尽管它们对水稻和向日葵等主要作物的生产以及植物细胞器/核相互作用的研究都很重要，但以前还没有克隆出减少异常线粒体蛋白表达的核育性恢复基因。在这里，我们报道了一个直接参与控制细胞质雄性不育编码基因表达的基因的分离。矮牵牛育性基因恢复产物是一种线粒体靶向蛋白，几乎完全由14个35-aa五肽重复基序组成。在一个非恢复基因型中，我们发现了一个同源基因，该基因在启动子区域缺失，在根中表达，而在花中不表达。
Known in over 150 species, cytoplasmic male sterility is encoded by aberrant mitochondrial genes that prevent pollen development. The RNA- or protein-level expression of most of the mitochondrial genes encoding cytoplasmic male sterility is altered in the presence of one or more nuclear genes called restorers of fertility that suppress the male-sterile phenotype. Cytoplasmic male sterility/restorer systems have been proven to be an invaluable tool in the production of hybrid seeds. Despite their importance for both the production of major crops such as rice and sunflower and the study of organelle/nuclear interactions in plants, none of the nuclear fertility-restorer genes that reduce the expression of aberrant mitochondrial proteins have previously been cloned. Here we report the isolation of a gene directly involved in the control of the expression of a cytoplasmic male sterility-encoding gene. The Petunia restorer of fertility gene product is a mitochondrially targeted protein that is almost entirely composed of 14 repeats of the 35-aa pentatricopeptide repeat motif. In a nonrestoring genotype we identified a homologous gene that exhibits a deletion in the promoter region and is expressed in roots but not in floral bus.