SYNTHESIS OF KALLIKREINS BY RAT KIDNEY SLICES
SYNTHESIS OF KALLIKREINS BY RAT KIDNEY SLICES
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通过大鼠肾切片合成激肽释放酶
DOI：
10.1111/j.1476-5381.1975.tb07353.x
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发表时间：
1975
影响因子：
7.3
通讯作者：
J. Pierce
中科院分区：
文献类型：
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作者：
K. Nustad;KIRSTEN VAAJE;J. Pierce
1从[H]-L-亮氨酸孵育的大鼠肾切片中分离到4种放射性激肽释放酶。2用先前用于分离大鼠尿激肽释放酶的程序(Nustad&Piells，1974)和亲和层析法纯化激肽释放酶。3肾激肽释放酶与尿激肽释放酶在相对含量、等电点和聚丙烯酰胺凝胶上的电泳率上与尿激肽释放酶相似。4肾脏合成四种激肽释放到尿液中。激肽释放酶不会因为通过下尿路而改变。
1 Four radioactive kallikreins were isolated from rat kidney slices incubated with [3 H]‐L‐leucine. 2 The kallikreins were purified by procedures previously used for the isolation of rat urinary kallikreins (Nustad & Pierce, 1974), and by affinity chromatography on a column of insolubilized anti‐rat urinary kallikrein. 3 The kidney kallikreins resembled the urinary kallikreins in their relative amounts, isoelectric points and electrophoretic mobilities on polyacrylamide disc gels. 4 The data indicate that the kidney synthesizes four kallikreins which are released into urine. The kallikreins are not changed by passage through the lower urinary tract.