RAPID EXPRESSION OF HEAT-SHOCK PROTEIN IN THE RABBIT AFTER BRIEF CARDIAC ISCHEMIA
RAPID EXPRESSION OF HEAT-SHOCK PROTEIN IN THE RABBIT AFTER BRIEF CARDIAC ISCHEMIA
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DOI：
10.1172/jci114963
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发表时间：
1991-01-01
影响因子：
15.9
通讯作者：
APSTEIN, CS
中科院分区：
文献类型：
--
作者：
KNOWLTON, AA;BRECHER, P;APSTEIN, CS
采用北方印迹法，观察了短暂心肌缺血对热休克蛋白70（HSP 70）表达的影响。 在开胸麻醉家兔中进行功能研究。 左回旋支的大边缘分支闭塞4次，持续5分钟。使用植入在缺血区中壁的压电晶体，舒张末期长度，收缩末期长度和节段缩短百分比进行了评估。 北方印迹法检测HSP 70的表达。 单次冠状动脉阻断5 min使HSP 70的表达增加一倍，而4次5 min缺血/5 min再灌注循环导致HSP 70 mRNA增加3倍（P < 0.001）。压电晶体的测量结果显示，随着HSP 70的增加，轻度心肌功能障碍。 反复短暂缺血后HSP 70 mRNA的这种增加是短暂的，早在缺血后1小时就出现，缺血后24小时恢复到基线水平。 用HSP 70单克隆抗体进行Western印迹分析，比较假手术和缺血后心肌HSP 70水平。 HSP 70含量的变化早在2 h就已明显，24 h时更为显著。
The effect of brief myocardial ischemia on the expression of heat shock protein (HSP 70) was examined in an in vivo rabbit model of myocardial ischemia using Northern blotting. Functional studies were carried out in the open-chested anesthetized rabbit. The large marginal branch of the left circumflex was occluded four times for 5 min. Using piezoelectric crystals implanted midwall in the ischemic zone, end-diastolic length, end-systolic length, and percent segmental shortening were assessed. Expression of HSP 70 was measured by Northern blotting. A single 5-min coronary occlusion doubled the expression of HSP 70 whereas four cycles of 5 min of ischemia/5 min of reperfusion resulted in a three fold increase in HSP 70 mRNA (P < 0.001). Measurements with the piezoelectric crystals showed mild myocardial dysfunction concomitant with the increase in HSP 70. This increase in HSP 70 mRNA after repetitive brief ischemia was transient, occurring as early as 1 h and returning to baseline by 24 h after ischemia. Western blot analysis with a monoclonal antibody to HSP 70 was used to compare sham and postischemic myocardial HSP 70 levels. Changes in the amount of HSP 70 were evident as early as 2 h and were even more striking at 24 h.