Study of the expression of myelin proteolipid protein (lipophilin) using a cloned complementary DNA.
Study of the expression of myelin proteolipid protein (lipophilin) using a cloned complementary DNA.
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使用克隆的互补 DNA 研究髓磷脂蛋白脂质蛋白（亲脂蛋白）的表达。
DOI：
10.1093/nar/13.20.7413
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发表时间：
1985
影响因子：
14.9
通讯作者：
John R Riordan
中科院分区：
文献类型：
--
作者：
A. Naismith;E. Hoffman;L. Tsui;John R Riordan
我们用从活跃髓鞘形成的胎牛脑中分离的mRNA制备了一个lambda gt10 cDNA文库。利用已知的髓磷脂蛋白脂质蛋白（PLP或lipphilin）氨基酸序列制备的两个寡核苷酸，我们分离出了髓磷脂主要内在膜蛋白的cDNA克隆。其中一个克隆被命名为pLP1，它含有444 bp的PLP c端编码序列和486 bp的3'未翻译序列。我们利用pLP1作为杂交探针，从mRNA水平研究了PLP在大鼠脑发育过程中的调控作用。我们的研究结果表明，在脑髓鞘形成过程中，PLP mRNA的相对量与髓鞘膜的主要外源蛋白（髓鞘碱性蛋白）mRNA的相对量同步增加。
We have prepared a lambda gt10 cDNA library with the mRNA isolated from fetal calf brains which were actively myelinating. Using two oligonucleotides made according to the known amino acid sequence of myelin proteolipid protein (PLP or lipophilin), we have isolated several cDNA clones for this major intrinsic membrane protein of myelin. One of these clones, designated as pLP1, is found to contain 444 bp of coding sequence for the C-terminal half of PLP and 486 bp of 3' untranslated sequence. Using pLP1 as a hybridization probe, we have studied the regulation of PLP at the mRNA level during rat brain development. Our results show that the relative amounts of mRNA for PLP and that for the major extrinsic protein of the myelin membrane, myelin basic protein, increase coordinately during the course of myelination in the brain.