Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization
Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization
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DOI：
10.1091/mbc.9.12.3273
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发表时间：
1998-12-01
影响因子：
3.3
通讯作者：
Futcher, B
中科院分区：
文献类型：
--
作者：
Spellman, PT;Sherlock, G;Futcher, B
我们试图创建酵母基因的全面目录，其转录物水平在细胞周期内定期变化。为此，我们使用了通过三种独立方法同步的酵母培养物中的DNA微阵列和样品：α因子停滞，洗脱和抑制Cdc15温度敏感的突变体。使用周期性和相关算法，我们确定了800个基因，这些基因符合细胞周期调节的客观最低标准。在单独的实验中，旨在检查诱导G1细胞周期蛋白CLN3P或B型细胞周期蛋白CLB2P的效果，我们发现这些800个基因中有一半以上的mRNA水平对一个或两个细胞周期蛋白有反应。此外，我们分析了已知和新启动子元素的细胞周期调节基因集，并表明几个已知元素（或其变异）包含可预测细胞周期调节的信息。可以在http://cellcycle-www.stanford.edu上获得完整的描述和完整的数据集。
We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: alpha factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu.