In vivo characterization of lung morphology and function in anesthetized free-breathing mice using micro-computed tomography
In vivo characterization of lung morphology and function in anesthetized free-breathing mice using micro-computed tomography
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DOI：
10.1152/japplphysiol.00629.2006
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发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
Holdsworth, D. W.
中科院分区：
文献类型：
--
作者：
Ford, N. L.;Martin, E. L.;Holdsworth, D. W.
人类受试者的肺形态和功能可以通过计算机断层扫描（CT）进行监测。由于许多人类呼吸系统疾病通常是在啮齿动物中建模的，因此需要一种监测啮齿动物肺部结构和功能变化的方法。啮齿动物肺部的高分辨率图像可以通过专门的微型 CT 设备获得，这提供了一种利用临床相关方法无创监测啮齿动物肺部疾病模型的方法。先前的研究已经显示了插管和呼吸小鼠的呼吸门控图像。尽管这些研究中的图像质量和分辨率足以进行定量测量，但肺结构的这些测量将取决于呼吸机的设置，而不是个体动物的呼吸力学。此外，插管和通气可能会对动物的呼吸动力学产生不自然的影响，因为气道压力、潮气量和呼吸频率是由操作员选择的。在这些实验中，由于呼吸被迫符合呼吸机设置，因此可能会错过有关正在研究的呼吸系统疾病症状的重要信息。在这项研究中，我们实施了一种呼吸门控微型 CT 方法，用于麻醉自由呼吸的啮齿动物。根据显微 CT 图像，对健康无意识小鼠的肺部结构进行定量分析，以获得气道直径、肺和气道体积以及呼气末和吸气时的 CT 密度。由于动物自由呼吸，我们能够计算潮气量（0.09 +/- 0.03 ml）和功能残气量（0.16 +/- 0.03 ml）。
Lung morphology and function in human subjects can be monitored with computed tomography (CT). Because many human respiratory diseases are routinely modeled in rodents, a means of monitoring the changes in the structure and function of the rodent lung is desired. High-resolution images of the rodent lung can be attained with specialized micro-CT equipment, which provides a means of monitoring rodent models of lung disease noninvasively with a clinically relevant method. Previous studies have shown respiratory-gated images of intubated and respirated mice. Although the image quality and resolution are sufficient in these studies to make quantitative measurements, these measurements of lung structure will depend on the settings of the ventilator and not on the respiratory mechanics of the individual animals. In addition, intubation and ventilation can have unnatural effects on the respiratory dynamics of the animal, because the airway pressure, tidal volume, and respiratory rate are selected by the operator. In these experiments, important information about the symptoms of the respiratory disease being studied may be missed because the respiration is forced to conform to the ventilator settings. In this study, we implement a method of respiratory-gated micro-CT for use with anesthetized free-breathing rodents. From the micro-CT images, quantitative analysis of the structure of the lungs of healthy unconscious mice was performed to obtain airway diameters, lung and airway volumes, and CT densities at end expiration and during inspiration. Because the animals were free breathing, we were able to calculate tidal volume (0.09 +/- 0.03 ml) and functional residual capacity (0.16 +/- 0.03 ml).