Bacteriophage involvement in group A streptococcal pyrogenic exotoxin A production
Bacteriophage involvement in group A streptococcal pyrogenic exotoxin A production
复制标题
噬菌体参与 A 组链球菌热原性外毒素 A 的产生
DOI：
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发表时间：
1986
影响因子：
3.2
通讯作者：
P. Schlievert
中科院分区：
文献类型：
--
作者：
L. Johnson;M. Tomai;P. Schlievert
溶原性转化被认为是控制A组链球菌产热性外毒素A型的一种机制。用多种限制性内切酶消化来自两种转化噬菌体3GL 16和T12的DNA，在琼脂糖凝胶中电泳时产生相同的DNA片段。分析了几种已知的A毒素阳性菌株的毒素和噬菌体DNA，这些菌株在诱导后似乎不产生转化噬菌体。杂交研究表明，包括NY5、594和C203S在内的菌株在染色体插入的噬菌体片段附近携带A毒素基因（speA），与噬菌体T12 DNA同源，这可能代表有缺陷的转化酶。噬菌体T12 att位点定位在speA附近。这些数据表明，噬菌体T12从细菌基因组中获得了A毒素基因。所有经Ouchterlony免疫扩散法检测为A毒素阴性的链球菌菌株均未显示与speA特异性探针的任何杂交。
Lysogenic conversion has been suggested as a mechanism of control of group A streptococcal pyrogenic exotoxin type A production. Digestion of DNA from two converting bacteriophages, 3GL16 and T12, with a variety of restriction endonucleases yielded identical DNA fragments upon electrophoresis in agarose gels. Several known A toxin-positive strains that did not appear to produce converting phage upon induction were analyzed for toxin and phage DNA. Strains, including NY5, 594, and C203S, were shown by hybridization studies to carry the A toxin gene (speA) adjacent to chromosomally inserted phage fragments, homologous to phage T12 DNA, which may represent defective converting phages. The phage T12 att site mapped adjacent to speA. These data suggest that phage T12 acquired the A toxin gene from the bacterial genome. All streptococcal strains tested that were A toxin negative by Ouchterlony immunodiffusion failed to show any hybridization to speA-specific probes.
A 组链球菌噬菌体 SP24 中的 DNA 取代。
影响因子：
3.7
作者：
Spanier,JG;Cleary,PP
通讯作者：
Cleary,PP