Preparation of a Beverage Containing Freeze-Dried Watercress for a Clinical Trial of Carcinogen and Toxicant Detoxification.
Preparation of a Beverage Containing Freeze-Dried Watercress for a Clinical Trial of Carcinogen and Toxicant Detoxification.
复制标题
DOI：
10.1158/1940-6207.capr-21-0473
复制
发表时间：
2022-03-01
期刊：
影响因子：
--
通讯作者：
Hecht SS
中科院分区：
文献类型：
--
作者：
Bonorden MJL;Carmella SG;Ballinger OT;Williams J;Dorn I;Vanderloo H;Fujioka N;Hatsukami DK;Hecht SS
西洋菜是异硫氰酸苯乙酯（PEITC）的极好来源，许多研究表明，它可以增强致癌物和有毒物的解毒作用，并抑制致癌作用。基于先前的观察，PEITC可以增强常见的环境污染物，如丙烯醛，巴豆醛，苯，和1，3-丁二烯的解毒，我们设计了一项临床试验，测试假设，每天饮用含有冻干豆瓣菜的饮料，PEITC的丰富来源，将有类似的效果，特别是在某些谷胱甘肽S-转移酶基因为空的受试者中观察到。这份手稿描述了为这项试验准备近100磅冻干豆瓣菜，从实验室规模的中试研究开始，并按照所有食品安全要求进行完全验证产品的工业规模生产。还提供了验证临床试验中受试者依从性的初步结果。
Watercress is an excellent source of phenethyl isothiocyanate (PEITC), shown in many studies to enhance carcinogen and toxicant detoxification and to inhibit carcinogenesis. Based on a previous observation that PEITC can enhance the detoxification of common environmental pollutants such as acrolein, crotonaldehyde, benzene, and 1,3-butadiene, we designed a clinical trial testing the hypothesis that daily consumption of a drink containing freeze-dried watercress, an abundant source of PEITC, would have a similar effect, particularly observed in subjects who were null in certain glutathione S-transferase genes. This manuscript describes the preparation of nearly 100 pounds of freeze-dried watercress for this trial, starting with laboratory scale pilot studies and proceeding to industrial scale production of the fully validated product in compliance with all food safety requirements. Initial results validating subject compliance in the clinical trial are also presented.