The expression of prion protein by endothelial cells: a source of the plasma form of prion protein?
The expression of prion protein by endothelial cells: a source of the plasma form of prion protein?
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内皮细胞表达朊病毒蛋白：血浆形式朊病毒蛋白的来源？
DOI：
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发表时间：
2002
影响因子：
6.5
通讯作者：
P. Harrison
中科院分区：
文献类型：
--
作者：
R. Starke;O. Drummond;I. Macgregor;J. Biggerstaff;R. Gale;R. Camilleri;I. Mackie;S. Machin;P. Harrison
总结。神经元朊蛋白（PrPC）也在包括人血液在内的外周组织中表达。大部分的PrPC存在于血浆中。我们假设血管内皮可能是这种PrPC的来源。逆转录聚合酶链反应表明，人脐静脉内皮细胞（HUVEC）和人微血管内皮细胞（HMEC‐1）均表达PrPC mRNA。流式细胞术证实PrPC在HMEC‐1s和HUVECs上表达（分别为120 900±15 058和58 327±4577分子/细胞），细胞激活后没有上调。共聚焦免疫荧光显微镜证实HMEC - 1s和HUVECs质膜上PrPC阳性。细胞培养基的时间分辨解离增强荧光免疫分析（DELFIA®）显示可溶性PrPC的缓慢组成释放与激活无关。与血管性血友病因子抗原相比，去氨加压素治疗后血管性血友病患者体内血浆PrPC水平降低。在不同血细胞计数的患者中测量PrPC血浆水平显示细胞计数和PrPC浓度之间没有关联。然而，终末期肾衰竭患者血浆中PrPC水平较高。综上所述，大血管和微血管内皮细胞均表达了高水平的PrPC， PrPC可以组成性地释放到细胞培养基中。
Summary. The neuronal prion protein (PrPC) is also expressed within peripheral tissues including human blood. The majority of blood PrPC is found within the plasma fraction. We hypothesized that the vascular endothelium could be a source of this PrPC. Reverse transcription polymerase chain reaction demonstrated that both human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC‐1) expressed PrPC mRNA. Flow cytometry confirmed PrPC expression on HMEC‐1s and HUVECs (120 900 ± 15 058 and 58 327 ± 4577 molecules PrPC/cell respectively), with no upregulation following cellular activation. Confocal immunofluorescence microscopy confirmed that HMEC‐1s and HUVECs were positive for PrPC on the plasma membrane. Time‐resolved dissociation‐enhanced fluoroimmunoassay (DELFIA®) analysis of cell culture medium demonstrated a slow constitutive release of soluble PrPC not associated with activation. In contrast to von Willebrand factor antigen, PrPC plasma levels in vivo decrease following desmopressin therapy in patients with von Willebrand disease. Measurement of PrPC plasma levels in patients with varying blood counts demonstrated no association between cell count and PrPC concentration. However, there was a higher level of PrPC in plasma from patients with end‐stage renal failure. In conclusion, endothelial cells of both macrovascular and microvascular origin expressed high levels of PrPC which can be constitutively released into the cell culture medium.
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PrP27-30 是人血小板释放的正常可溶性朊病毒蛋白片段。
DOI：
10.1006/bbrc.1996.0936
发表时间：
1996
期刊：
Biochemical and biophysical research communications.
影响因子：
--
作者：
Perini,F;Vidal,R;Ghetti,B;Tagliavini,F;Frangione,B;Prelli,F
通讯作者：
Prelli,F
影响因子：
4.3
作者：
Li, RL;Liu, DC;Sy, MS
通讯作者：
Sy, MS
影响因子：
5
作者：
Satoh, J.;Obayashi, S.;Tabunoki, H.
通讯作者：
Tabunoki, H.
荧光流式细胞术证明血小板分泌对凝血酶的异质性。
影响因子：
20.3
作者：
Johnston,GI;Pickett,EB;McEver,RP;George,JN
通讯作者：
George,JN