Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging
Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging
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DOI：
10.1007/978-981-33-6064-8_10
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发表时间：
2021-01-01
期刊：
影响因子：
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通讯作者：
Park, YongKeun
中科院分区：
文献类型：
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作者：
Kim, Doyeon;Lee, Sangyun;Park, YongKeun
活细胞成像为细胞生物学和相关病理生理学研究提供了重要信息。折射率 (RI) 可作为 3D 无标记和定量活细胞成像的内在光学成像对比度，并为了解细胞和组织的各种动态以供众多领域的研究提供宝贵的信息。最近，利用 RI 的成像方法和分析方法取得了重大进展，现已转移到生物和医学研究领域，为研究细胞病理生理学提供了新方法。为了深入了解 RI 如何用作生物标本成像的成像对比，这里我们提供了基于 RI 的成像技术的基本原理，并总结了微生物学、血液学、传染病、血液学和组织病理学等应用的最新进展。
Live cell imaging provides essential information in the investigation of cell biology and related pathophysiology. Refractive index (RI) can serve as intrinsic optical imaging contrast for 3-D label-free and quantitative live cell imaging, and provide invaluable information to understand various dynamics of cells and tissues for the study of numerous fields. Recently significant advances have been made in imaging methods and analysis approaches utilizing RI, which are now being transferred to biological and medical research fields, providing novel approaches to investigate the pathophysiology of cells. To provide insight into how RI can be used as an imaging contrast for imaging of biological specimens, here we provide the basic principle of RI-based imaging techniques and summarize recent progress on applications, ranging from microbiology, hematology, infectious diseases, hematology, and histopathology.