Blockage of progestin physiology disrupts ovarian differentiation in XX Nile tilapia (Oreochromis niloticus).
Blockage of progestin physiology disrupts ovarian differentiation in XX Nile tilapia (Oreochromis niloticus).
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DOI：
10.1016/j.bbrc.2016.03.045
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发表时间：
2016-04
影响因子：
3.1
通讯作者：
Linyan Zhou;Feng Luo;Xuelian Fang;Tapas Charkraborty;Limin Wu;Jing Wei;Deshou Wang
中科院分区：
文献类型：
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作者：
Linyan Zhou;Feng Luo;Xuelian Fang;Tapas Charkraborty;Limin Wu;Jing Wei;Deshou Wang
成熟诱导激素17α，20β-二羟基-4-异戊烯-3-酮（DHP）可能通过核受体（Pgr）参与鱼类精子发生和卵子发生。为了进一步阐明DHP在硬骨鱼卵巢分化中的作用，我们分析了Pgr在尼罗罗非鱼（Oreochromis niloticus）卵巢中的表达，并对XX鱼从孵化后5天（dah）到120天（dah）进行了RU 486（一种合成的Pgr拮抗剂）处理。30和90日龄时，Pgr在卵母细胞周围的滤泡细胞中大量表达。连续RU 486处理导致XX鱼卵子发生受阻和体细胞雄性化。终止RU 486处理并维持在正常条件下导致睾丸分化，并且RU 486处理的XX鱼中的雌激素补偿成功地恢复卵子发生。在RU 486处理的XX鱼中，雌性显性基因的转录水平显著降低，而雄性偏向基因的转录水平明显增加。同时，生殖细胞有丝分裂和减数分裂标记均显著减少。一致的是，雌激素的生产水平显着下降，在RU 486处理XX鱼。综上所述，我们的数据进一步证明，DHP，可能通过Pgr，可能是必需的卵巢分化和雌激素的产生在鱼类。
Previous studies indicated that maturation inducing hormone, 17α, 20β-Dihydroxy-4-pregnen-3-one (DHP), probably through nuclear progestin receptor (Pgr), might be involved in spermatogenesis and oogenesis in fish. To further elucidate DHP actions in teleostean ovarian differentiation, we analyzed the expression ofpgrin the ovary of Nile tilapia (Oreochromis niloticus), and performed RU486 (a synthetic Pgr antagonist) treatment in XX fish from 5 days after hatching (dah) to 120dah. Tilapia Pgr was abundantly expressed in the follicular cells surrounding oocytes at 30 and 90dah. Continuous RU486 treatment led to the blockage of oogenesis and masculinization of somatic cells in XX fish. Termination of RU486 treatment and maintenance in normal condition resulted in testicular differentiation, and estrogen compensation in RU486-treated XX fish successfully restored oogenesis. In RU486-treated XX fish, transcript levels of female dominant genes were significantly reduced, while male-biased genes were evidently augmented. Meanwhile, both germ cell mitotic and meiotic markers were substantially reduced. Consistently, estrogen production levels were significantly declined in RU486-treated XX fish. Taken together, our data further proved that DHP, possibly through Pgr, might be essential in the ovarian differentiation and estrogen production in fish.