White Button Mushroom, Agaricus bisporus (Agaricomycetes), and a Probiotics Mixture Supplementation Correct Dyslipidemia without Influencing the Colon Microbiome Profile in Hypercholesterolemic Rats
White Button Mushroom, Agaricus bisporus (Agaricomycetes), and a Probiotics Mixture Supplementation Correct Dyslipidemia without Influencing the Colon Microbiome Profile in Hypercholesterolemic Rats
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DOI：
10.1615/intjmedmushrooms.2020033807
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Sohail, Muhammad Umar
中科院分区：
文献类型：
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作者：
Asad, Farhan;Anwar, Haseeb;Sohail, Muhammad Umar
通过调节肠道微生物组来降低血液胆固醇和甘油三酯，富含膳食纤维的食物的摄入已引起了相当大的关注。富含纤维的饮食可能为微生物提供消化和增殖的底物。相应地，微生物消化的产物进入体循环并支持宿主的能量稳态。在本研究中，给高胆固醇血症大鼠（TIC）补充益生菌（PB）和双孢蘑菇以检验其抗血脂异常的效果。40只成年大鼠被分为5个处理组。对照组的大鼠仅喂食基础维持饲料（CON；n = 8），而其余大鼠则给予致动脉粥样硬化饮食（基础饲料中添加1.5%胆固醇和0.5%胆酸）以诱导高胆固醇血症（HC组；n = 32）。大鼠在连续24天给予致动脉粥样硬化饮食后出现高胆固醇血症。从第25天起，HC组进一步分为HC - CON、HC - PB（以1毫克/只/天的剂量补充PB）、HC - AB（在饲料中添加5%的双孢蘑菇）和HC - AB.PB（同时补充双孢蘑菇和PB）。补充6周后，处死大鼠采集血液以测定血脂谱、氧化应激，并对结肠内容物进行宏基因组学分析。结果表明，所有补充剂都纠正了高胆固醇血症诱导的氧化应激。此外，双孢蘑菇补充剂纠正了高胆固醇血症诱导的血脂异常（P
Consumption of foods rich in dietary fiber has attracted considerable attention for lowering blood cholesterol and triglycerides through attenuation of gut microbiome. Diets rich in fiber may provide substrates for microbes to digest and proliferate. In response, products of microbial digestion enter systemic circulation and support host energy homeostasis. In the present study, rats with hypercholesterolemia (TIC) were supplemented with probiotics (PB) and Agaricus bisporus mushroom to examine the antidyslipidemia effects. Forty adult rats were divided into five treatment groups. The rats in the control group were fed only a chow maintenance diet (CON; n = 8), whereas an atherogenic diet (chow diet supplemented with 1.5% cholesterol and 0.5% cholic acid) was offered to the remaining rats to induce hypercholesterolemia (HC group; n = 32). Rats developed HC following a 24-day continuous supplementation with the atherogenic diet. From day 25 onward, the HC group was further divided into HC-CON, HC-PB (supplemented with PB at 1 mg/rat/day), HC-AB (supplemented with A. bisporus at 5% of diet), and HC-AB.PB (supplemented with both A. bisporus and PB). After 6 weeks of supplementation, rats were killed to collect blood to determine serum lipid profile, oxidative stress, and for metagenomics analysis of colon contents. Results showed that all supplementations corrected HC-induced oxidative stress. Furthermore, A. bisporus supplementation corrected HC-induced dyslipidemia (P