Bacteriology of chronic suppurative otitis media (CSOM) in children in Garissa district, Kenya: A point prevalence study
Bacteriology of chronic suppurative otitis media (CSOM) in children in Garissa district, Kenya: A point prevalence study
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DOI：
10.1016/j.ijporl.2013.04.011
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发表时间：
2013-07-01
影响因子：
1.5
通讯作者：
Mackenzie, Ian
中科院分区：
文献类型：
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作者：
Aduda, Dickens S. O.;Macharia, Isaac M.;Mackenzie, Ian
目的：要确定的类型和药物的敏感性，发现在耳朵里的上学的孩子与慢性中耳炎Garissa district.Methods的细菌：研究设计：这是一个描述性的患病率研究CSOM细菌植物群在合格的耳朵中进行了一个队列的儿童参加公立和私立小学以及伊斯兰宗教学校，筛选慢性耳分泌物在加里萨区，肯尼亚。程序和细菌学技术：在2010年1月至7月进行的治疗慢性化脓性中耳炎的补锌试验的诱导阶段，我们使用无菌拭子棒从同意的小学生的合格耳朵中收集分泌物样本。所有在访问当天在场的18岁以下的学生都有资格。进行需氧和厌氧细菌培养以鉴定临床和流行病学上重要的细菌。敏感性试验基于纸片扩散法。结果呈现为频率和proportions.Results：看到的学生，61%仍在学前或小学低年级。大多数是13和14岁。在处理的261份耳拭子样本中，鉴定了336株分离株-混合或纯植物群，几乎全部为需氧菌。变形杆菌属，肠球菌、金黄色葡萄球菌和假单胞菌属。分离率分别为32.7%、28.6%、12.8%和11.3%。变形杆菌属对大多数抗生素敏感，而肠球菌属的敏感性较差。结论：需氧菌在本研究中最普遍。已知几种被鉴定的细菌需要铁来生长。如果生物膜形成参与发病机制，这对于CSOM治疗可能是重要的。与肠球菌属细菌相比，大多数分离株对碱性抗生素敏感。这预示着临床管理和治疗决策的重要考虑因素。此外，考虑到肠球菌的流行，这是环境粪便污染的一个指标，在管理儿童CSOM时，除了单独的临床治疗外，还需要考虑相关的公共卫生因素。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Objectives: To identify by type and sensitivity to drugs the bacteria found in ears of school-going children with chronic otitis media in Garissa district.Methods: Study design: This was a descriptive prevalence study of CSOM bacterial flora in eligible ears conducted among a cohort of children attending public and private primary as well as Islamic religious schools, screened for chronic ear discharge in Garissa district, Kenya. Procedure and bacteriological techniques: We used sterile swab-sticks to collect a specimen of the discharge from eligible ears of consenting pupils at the induction stage of the zinc supplementation trial for treatment of chronic suppurative otitis media conducted between January and July 2010. All pupils below 18 years present on day of visit were eligible. Both aerobic and anaerobic bacterial cultures were done to identify clinically and epidemiologically important bacteria. Sensitivity tests were based on disc diffusion methods. Results are presented as frequencies and proportions.Results: Of the pupils seen, 61% were still in pre- or lower primary school. Majority were aged 13 and 14 years. Of the 261 ear swab samples processed, 336 isolates - either in mixed or pure flora were identified, being almost exclusively aerobes. Proteus spp., Enterococcus, Staphylococcus aureus and Pseudomonas spp. were isolated in 32.7%, 28.6%, 12.8% and 11.3% respectively. Proteus was susceptible to majority of the antibiotics tested for, while Enterococcus was poorly susceptible.Conclusions: Aerobic bacteria were most prevalent in this study. Several of the bacteria identified are known to require iron for their growth. This may be important for CSOM treatment if biofilm formation is involved in pathogenesis. Majority of the isolates were susceptible to basic antibiotics compared to Enterococcus bacteria. This portends an important consideration for clinical management and therapeutic decision-making. Additionally, given the prevalence of Enterococcus bacteria, which is an indicator of faecal contamination of the environment, there is need to consider relevant public health components in managing childhood CSOM besides the clinical ones alone. (C) 2013 Elsevier Ireland Ltd. All rights reserved.