Inhibition of glutamatergic transmission by morphine in the basolateral amygdaloid nucleus reduces pain-induced aversion
Inhibition of glutamatergic transmission by morphine in the basolateral amygdaloid nucleus reduces pain-induced aversion
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DOI：
10.1016/j.neures.2007.06.1473
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发表时间：
2007-10-01
影响因子：
2.9
通讯作者：
Minami, Masabumi
中科院分区：
文献类型：
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作者：
Deyama, Satoshi;Yamamoto, Junki;Minami, Masabumi
我们研究了戊二酸能传递的作用，在基底外侧杏仁核（BLA）在疼痛引起的厌恶使用条件的地方范例和在体微透析技术在大鼠。在足底注射福尔马林前5 min，将MK-801（1或10 nmol/侧）微量注射到双侧BLA中，可剂量依赖性地减弱福尔马林诱导的条件性位置厌恶（F-CPA），而不影响伤害性行为，如抬起、舔和咬。相反，CNQX（30 nmol/侧）或AP-3（30 nmol/侧）的微量注射对F-CPA无显著影响。微透析实验表明，足底注射福尔马林引起的BLA内的细胞外谷氨酸水平的增加。通过微透析探针，吗啡灌注（100 μ M）抑制谷氨酸的增加。此外，内BLA注射吗啡（10 μ g/侧）5分钟前福尔马林注射衰减F-CPA不影响伤害性行为。这些结果表明，通过NMDA受体在BLA的谷氨酸能传输起着至关重要的作用，在疼痛引起的厌恶，以及除了良好的特征化的影响疼痛的感觉成分，吗啡也影响疼痛的情感成分通过抑制作用内BLA的谷氨酸能传输。(C)2007 Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
We examined the role of glutarnatergic transmission within the basolateral amygdaloid nucleus (BLA) in pain-induced aversion using a conditioned place paradigm and an in vivo microdialysis technique in rats. Microinjection of MK-801 (I or 10 nmol/side) into the bilateral BLA 5 min before intraplantar injection of formalin dose-dependently attenuated formalin-induced conditioned place aversion (F-CPA) without affecting nociceptive behaviors, such as lifting, licking, and biting. On the contrary, microinjection of neither CNQX (30 nmol/side) nor AP-3 (30 nmol/side) showed any significant effect on F-CPA. Microdialysis experiments revealed that intraplantar injection of formalin induced an increase in the extracellular glutamate level within the BLA. This increase in glutamate was suppressed by morphine perfusion (100 mu M) via the microdialysis probe. Moreover, intra-BLA injection of morphine (10 mu g/side) 5 min before formalin injection attenuated F-CPA without affecting nociceptive behaviors. These results suggest that glutarnatergic transmission via NMDA receptors in the BLA plays a crucial role in the pain-induced aversion, and that in addition to the well-characterized effects on the sensory component of pain, morphine also influences the affective component of pain through an inhibitory effect on intra-BLA glutamatergic transmission. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.