Relatedness structure estimated by microsatellites DNA and mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphisms analyses in the wild population of kuruma prawn Penaeus japonicus
Relatedness structure estimated by microsatellites DNA and mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphisms analyses in the wild population of kuruma prawn Penaeus japonicus
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日本对虾野生种群微卫星DNA和线粒体DNA聚合酶链反应-限制性片段长度多态性分析的相关性结构分析
DOI：
10.1046/j.1444-2906.2002.00495.x
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发表时间：
2002
影响因子：
1.9
通讯作者：
N. Taniguchi
中科院分区：
文献类型：
--
作者：
T. Sugaya;M. Ikeda;N. Taniguchi
通过估计个体间的亲缘关系，研究了日本4个野虾类群体的遗传结构和变异性。用5个微卫星DNA标记进行亲缘关系分析。相关性检验表明，在熊本和鹿儿岛，个体之间的相关性显著。在鹿儿岛，一些个体表现出全同胞水平的亲缘关系。线粒体（mt）DNA聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的分析也进行了，表明在鹿儿岛的密切相关的个人往往有一个共同的单倍型。因此，人们认为在熊本和鹿儿岛有许多亲戚。然而，在MS-DNA分析的杂合性和等位基因和基因型频率没有显着差异的地方。此外，熊本和鹿儿岛的mtDNA单倍型分布与其他地区有显著差异。因此，它建议，没有发生空间分化，由于地理或历史的影响之间的地方，并有可能在熊本和鹿儿岛的孵化种群的混合物。
: The genetic structure and variability of four wild populations of kuruma prawn in Japan were examined by estimating relatedness among individuals. The relatedness was estimated by five microsatellites (MS) DNA markers. Examination of relatedness showed that individuals were related significantly in Kumamoto and Kagoshima. In Kagoshima, some individuals showed full-sib level of relatedness. The analysis of mitochondrial (mt)DNA polymerase chain reaction–restriction fragment length polymorphisms (PCR-RFLP) was also performed, showing that the closely related individuals in Kagoshima tended to share a common haplotype. It is, therefore, supposed that there are many kins in Kumamoto and Kagoshima. However, the heterozygosities and allelic and genotypic frequencies in MS-DNA analysis were not significantly different among the localities. Moreover, the haplotype distributions of mtDNA in Kumamoto and Kagoshima were significantly different from other localities. Thus, it is suggested that no spatial differentiations occurred due to the geographic or historical effects between the localities and that there is the possibility of a mixture of hatchery populations in Kumamoto and Kagoshima.