[Effect of moxibustion at "Zusanli"(ST36) on metabolites of gastric tissue in rats with chronic atrophic gastritis based on metabonomics].
[Effect of moxibustion at "Zusanli"(ST36) on metabolites of gastric tissue in rats with chronic atrophic gastritis based on metabonomics].
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DOI：
10.13702/j.1000-0607.180149
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发表时间：
2019-02-25
期刊：
影响因子：
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通讯作者：
Chang, Xiao-Rong
中科院分区：
文献类型：
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作者：
Liu, Xia;She, Chang;Chang, Xiao-Rong
目的：观察艾灸“足三里”穴对慢性萎缩性胃炎(CAG)大鼠胃组织代谢产物的影响，从代谢角度探讨阳明经与胃的相关性。方法：将56只SD大鼠随机分为正常组、正常+艾灸组、模型组和模型+艾灸组(n=14只/组)。以N-甲基-N‘-硝基-N-亚硝基-胍(MNNG)溶液灌胃，不规律饮食12周，建立CAG模型。正常+艾灸组和模型+艾灸组应用艾灸ST36、中湾穴15min，1次/d，连续2周。HE染色观察大鼠胃组织病理变化。结果：造模后，模型+艾灸组大鼠局部胃壁苍白变薄，腺体减少，坏死排列紊乱，上皮细胞脱落，淋巴细胞浸润，散在点状出血，明显减轻。模型组大鼠胃组织中乳酸、谷胱甘肽、N-乙酰天冬氨酸、尿苷二磷酸葡萄糖和肌糖的代谢物均高于正常组(P
OBJECTIVE: To observe the effect of moxibustion at "Zusanli" (ST36) on metabolites of gastric tissue in chronic atrophic gastritis (CAG) rats, so as to explore the correlation between Yangming Meridian and stomach based on metabolomics.METHODS: Fifty-six SD rats were randomly divided into normal, normal ＋ moxibustion, model and model ＋ moxibustion groups (n＝14 rats/group). The CAG model was established by gavage of N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) solution and irregular diet for 12 weeks. Moxibustion was applied to ST36 and "Zhongwan" (CV12) for 15 min, once daily for two weeks in the normal＋ moxibustion group and model＋moxibustion group. The histopathological changes of the gastric tissue were observed by H．E. staining. 1H nuclear magnetic resonance (1H NMR) spectroscopy was used to detect gastric tissue metabolites and the data were analyzed using the Chenomx NMR Suite software.RESULTS: After modeling, the regional gastric tissue showed pale thinner gastric wall, and reduction of regional glands with necrosis and disordered arrangement, epithelial cell exfoliation, lymphocyte infiltration as well as scattered punctate hemorrhage, which was evidently milder in the model ＋ moxibustion group. The metabolites of lactic acid, glutathione, N-acetylaspartate, uridine diphosphate glucose and muscle sugar in the gastric tissue in the model group were increased compared with those in the normal group (P