Hypothalamic Agouti-Related Protein Expression Is Affected by Both Acute and Chronic Experience of Food Restriction and Re-Feeding in Chickens
Hypothalamic Agouti-Related Protein Expression Is Affected by Both Acute and Chronic Experience of Food Restriction and Re-Feeding in Chickens
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DOI：
10.1111/jne.12088
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发表时间：
2013-10-01
影响因子：
3.2
通讯作者：
Boswell, T.
中科院分区：
文献类型：
--
作者：
Dunn, I. C.;Wilson, P. W.;Boswell, T.
中枢黑皮质素系统在脊椎动物中是保守的。然而，在鸟类中，很少有人知道能量平衡如何影响orexigenic agouti-related protein（AGRP）和orexigenic pro-opiomelanocortin（POMC）的表达，尽管事实上，商业食品限制是家禽肉的有效生产的关键。在肉种鸡中，在食物限制水平之间、在具有相同体重但不同饲养经验的禽类之间以及在从限制饲养到自由饲养的禽类之间进行对比。在6 ~ 12周龄之间建立了5组不同的限食和解除限食组合的母鸡。AGRP和神经肽Y在基底下丘脑的表达显着增加慢性限制，但只有AGRP mRNA水平反映了最近的喂养经验：母鸡在相同的体重，最近一直在自由。喂食显示比限制性鸟类低的表达。AGRP表达也区分母鸡从限制释放到自由。不同时期的喂养。相比之下，POMC和可卡因和安非他明调节的转录mRNA水平没有不同。这些结果表明，AGRP mRNA不仅反映了一只鸟的体重和它的潜在体重或设定点之间的差异，而且还区分了相同体重下不同的鸟类摄食历史。因此，AGRP表达潜在地提供了食物摄入经验的综合测量和评估用于改善家禽福利的饲养方案中的鸟的饱腹感的客观工具。
The central melanocortin system is conserved across vertebrates. However, in birds, little is known about how energy balance influences orexigenic agouti-related protein (AGRP) and anorexigenic pro-opiomelanocortin (POMC) expression, despite the fact that commercial food restriction is critical to the efficient production of poultry meat. To enable contrasts to be made, in broiler-breeder chickens, between levels of food restriction, between birds with the same body weight but different feeding experience, and between birds moved from restricted feeding to ad lib. feeding for different periods, five groups of hens were established between 6 and 12 weeks of age with different combinations of food restriction and release from restriction. AGRP and neuropeptide Y expression in the basal hypothalamus was significantly increased by chronic restriction but only AGRP mRNA levels reflected recent feeding experience: hens at the same body weight that had recently been on ad lib. feeding showed lower expression than restricted birds. AGRP expression also distinguished between hens released from restriction to ad lib. feeding for different periods. By contrast, POMC and cocaine- and amphetamine-regulated transcript mRNA levels were not different. These results showed that AGRP mRNA not only reflected differences between a bird's weight and its potential weight or set point, but also discriminated between differing feeding histories of birds at the same body weight. Therefore, AGRP expression potentially provides an integrated measure of food intake experience and an objective tool to assess a bird's perception of satiety in feeding regimes for improved poultry welfare.