Abdominal‐B contributes to abdominal identity in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
Abdominal‐B contributes to abdominal identity in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
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DOI：
10.1111/imb.12771
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发表时间：
2022-03
影响因子：
2.6
通讯作者：
Sun-Jie Chen;Xin-Yang Liu;Jing-li Zhang;Zhang-nv Yang;Hai-Jun Xu
中科院分区：
文献类型：
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作者：
Sun-Jie Chen;Xin-Yang Liu;Jing-li Zhang;Zhang-nv Yang;Hai-Jun Xu
同源异型复合体基因abd-B参与调节后腹部的发育，在全变态昆虫中得到了广泛的研究。然而，Abd-B在半代谢昆虫中的功能还不完全清楚。在这里，我们对褐飞虱(BPH)中的ABD-B同系物进行了功能鉴定。褐飞虱Abd-B同源基因(NlAbd-B)全长为2334个核苷酸，开放阅读框为1113个碱基。NlAbd-B在卵期的表达水平高于若虫期和成虫期，主要在胚胎的第4~9腹节表达。RNA干扰(RNAi)介导的NlAbd-B在若虫体内的敲除干扰了雌性和雄性生殖器的发育，并导致了生殖器到腿的转化。NlAbd-B在雌雄成虫中的亲本RNAi在后代若虫中引起额外的腹部节段，而褐飞虱腹型A同源基因在雌雄成虫中的亲本RNAi导致胚胎在第二至第八腹节上有腿状附属物。这些发现表明，NlAbd-B在生殖器发育和后腹部模式中起着关键作用，从而突出了Abd-B在全变态和半变态昆虫中的保守作用。
The homeotic complex gene Abdominal‐B (Abd‐B) is involved in regulating the development of posterior abdomens and has been extensively studied in holometabolous insects. However, the function of Abd‐B in hemimetabolous insects is not fully understood. Here, we functionally characterize an Abd‐B homologue in the brown planthopper (BPH), Nilaparvata lugens. The full‐length cDNA of the N. lugens Abd‐B homologue (NlAbd‐B) is 2334 nt, with an open reading frame of 1113 bp. NlAbd‐B has the highest expression level at the egg stage relative to the nymphal and adult stages and is mainly expressed in the fourth to the ninth abdominal segment of embryos. RNA interference (RNAi)‐mediated knockdown of NlAbd‐B in nymphs disrupted the development of genitalia both in females and males and caused a genitalia‐to‐leg transformation. Parental RNAi of NlAbd‐B in both female and male adults caused an extra abdominal segment in offspring nymphs, while parental RNAi of the N. lugens abdominal‐A homologue in both female and males adults led to embryos with leg‐like appendages on the second to the eighth abdominal segment. These findings suggest that NlAbd‐B plays a pivotal role in genital development and posterior abdominal patterning and thus highlight the conservational role of Abd‐B in holometabolous and hemimetabolous insects.