Enzymic Dissociation of Zea Shoot Cell Wall Polysaccharides : IV. Dissociation of Glucuronoarabinoxylan by Purified Endo-(1 --> 4)-beta-Xylanase from Bacillus subtilis.
Enzymic Dissociation of Zea Shoot Cell Wall Polysaccharides : IV. Dissociation of Glucuronoarabinoxylan by Purified Endo-(1 --> 4)-beta-Xylanase from Bacillus subtilis.
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玉米芽细胞壁多糖的酶解：IV。
DOI：
10.1104/pp.75.3.759
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发表时间：
1984
期刊：
影响因子：
7.4
通讯作者：
D. Nevins
中科院分区：
文献类型：
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作者：
Y. Kato;D. Nevins
玉米中一种葡萄糖醛酸阿拉伯木聚糖的结构。（杂种B73 × Mo 17）茎细胞壁的变化。将用纯化的枯草芽孢杆菌（1 -> 3）（1 -> 4）-β-d-葡聚糖4-葡聚糖水解酶预处理的玉米芽细胞壁的水不溶性部分用纯化的B处理。枯草内切-（1 -> 4）-β-木聚糖酶。酶处理产生的碳水化合物（占玉米芽细胞壁水不溶性部分的2.6%）由分子量从几百道尔顿到超过2.0 x 10（5）道尔顿不等的葡萄糖醛酸阿拉伯木聚糖片段组成。片段的结构分析表明，葡糖醛酸阿拉伯木聚糖具有木聚糖主链，该木聚糖主链含有（1 -> 4）-β-d-吡喃木糖残基，在C-2或C-3位置上约60 - 70%被阿拉伯糖、葡糖醛酸和其它取代基取代。此外，glucuronoarabinoxylan含有一种酚类成分，似乎主要是阿魏酸键合到碳水化合物，可能是通过酯键。阿魏酸的量为每100微克碳水化合物约3微克。
The structure of a glucuronoarabinoxylan in Zea mays L. (hybrid B73 x Mo17) shoot cell walls has been studied. The water-insoluble fraction of Zea shoot cell walls, pretreated with purified Bacillus subtilis (1 --> 3), (1 --> 4)-beta-d-glucan 4-glucanohydrolase, was treated with purified B. subtilis endo-(1 --> 4)-beta-xylanase. Carbohydrates (2.6% of the waterinsoluble fraction of Zea shoot cells walls) derived from the enzyme treatment consisted of glucuronoarabinoxylan fragments with molecular weights which varied from a few hundred to over 2.0 x 10(5) daltons. Structural analyses of the fragments suggested that the glucuronoarabinoxylan had a xylan backbone which contained (1 --> 4)-beta-d-xylopyranosyl residues, with about 60 to 70% substitution at the C-2 or C-3 position with arabinose, glucuronic acid, and other substituents. Furthermore, the glucuronoarabinoxylan contained a phenolic component which appeared to be primarily ferulic acid bonded to carbohydrate, probably by an ester linkage. The amount of ferulic acid was approximately 3 micrograms per 100 micrograms of carbohydrate.
具有 4-(1,3-苯并咪唑-2-基)-3-羟基苯基的二氧杂环丁烷的发光
DOI：
--
发表时间：
2007
期刊：
影响因子：
--
作者：
萩原啓雅;渡辺信子;松本正勝
通讯作者：
松本正勝