The effect of 3D-printed Ti6Al4V scaffolds with various macropore structures on osteointegration and osteogenesis: A biomechanical evaluation
The effect of 3D-printed Ti6Al4V scaffolds with various macropore structures on osteointegration and osteogenesis: A biomechanical evaluation
复制标题
具有各种大孔结构的3D打印Ti6Al4V支架对骨整合和成骨的影响：生物力学评估
DOI：
10.1016/j.jmbbm.2018.08.049
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发表时间：
2018-12-01
影响因子：
3.9
通讯作者：
Wang, Chao
中科院分区：
文献类型：
--
作者：
Wang, Han;Su, Kexin;Wang, Chao
正确设计的多孔支架可以加速骨整合过程，并且计算机辅助设计（CAD）和增材制造（AM）技术的使用有可能改进传统的多孔支架方法。在本研究中，我们评估了不同孔隙结构的多孔Ti6Al4V（Ti）对骨整合和成骨的影响。基于四种市售植入物，通过 CAD 和选择性激光熔化 (SLM) 设计和制造了具有不同孔结构的多孔钛支架。 Micro-CT表明SLM能够生产具有不同孔结构的钛支架。通过有限元分析和压缩测试评估的机械性能表明，我们研究中的四种多孔支架尽管机械性能不同，但在机械上进行了调整。然后，我们使用3D打印的多孔盘培养人骨髓间充质干细胞（hBMMSCs），这是骨组织工程的主要种子细胞。结果显示，四组细胞形态、活力和增殖能力无显着差异。此外，尽管 Ti-g 具有较高的碱性磷酸酶活性 (ALP)，但四组显示出相当的矿化能力。兔子模型的体内测试表明，所有四组都适合新骨的长入和整合。这些发现表明钛支架中的四种不同的孔结构提供了良好的骨整合和成骨作用。
A properly designed porous scaffold can accelerate the osseointegration process, and the use of computer-aided design (CAD) and additive manufacturing (AM) techniques has the potential to improve the traditional porous scaffold approach. In this study, we evaluate the effect of porous Ti6Al4V (Ti) with different pore structures on osteointegration and osteogenesis. Porous Ti scaffolds with different pore structures based on four commercially available implants were designed and manufactured by CAD and selective laser melting (SLM). Micro-CT showed that SLM was able to produce Ti scaffolds with different pore structures. The mechanical properties evaluated by finite element analysis and compression tests indicated that the four porous scaffolds in our study were mechanically adapted, despite their different mechanical properties. Then, we used 3D-printed porous discs to culture human bone marrow mesenchymal stem cells (hBMMSCs), the main seed cells of bone tissue engineering. The results showed no significant difference among the four groups in cell morphology, viability and proliferation. In addition, four groups showed a comparable mineralization ability even though Ti-g had a higher alkaline phosphatase activity (ALP). In vivo tests in a rabbit model showed that all four groups were suitable for new bone ingrowth and integration. These findings indicate that the four different pore structures in the Ti scaffolds provided good osteointegration and osteogenesis.