Characterization of the differential expression of uncoupling protein 2 and ROS production in differentiated mouse macrophage-cells (Mm1) and the progenitor cells (M1)
Characterization of the differential expression of uncoupling protein 2 and ROS production in differentiated mouse macrophage-cells (Mm1) and the progenitor cells (M1)
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DOI：
10.1007/s10735-004-2915-x
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发表时间：
2005-02-01
影响因子：
3.2
通讯作者：
Yamashita, H
中科院分区：
文献类型：
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作者：
Nishio, K;Qiao, SL;Yamashita, H
研究了未分化小鼠髓系白血病 (M1) 及其分化巨噬细胞样细胞 (Mm1) 中线粒体解偶联蛋白 2 (UCP2) 的表达状态。与未分化白血病细胞 (M1) 相比，Mm1 细胞具有高吞噬能力，并且产生显着高水平的活性氧 (ROS)、UCP2 蛋白和锰超氧化物歧化酶 (Mn-SOD)。尽管两种细胞类型中的 UCP2 mRNA 水平相似，但 Mm1 细胞表达的 UCP2 蛋白是未分化 M1 细胞的 10 倍。 Mm1 细胞中 UCP2 的较高表达表明 UCP2 在 ROS 产生中具有调节作用。此外，使用 MitoTracker Red CM-H(2)Xros 进行直接可视化显示，在 Mm1 细胞中转染 UCP2-GFP 表达载体会消除线粒体膜电位并减少 ROS 产生。巨噬细胞 gp91phox 蛋白是 NADPH 氧化酶复合物的膜催化成分，在 UCP2-GFP 表达和非表达的 Mm1 细胞中处于相似的水平。这些结果表明，未分化细胞的UCP2蛋白在相当低的水平上受到调节，而分化的巨噬细胞的较高UCP2蛋白参与ROS产生的调节。
The expression status of mitochondrial uncoupling protein 2 (UCP2) was investigated in undifferentiated mouse myeloid leukemia (M1) and its differentiated macrophage-like cells (Mm1). Mm1 cells have a high ability of phagocytosis along with significantly high levels of reactive oxygen species (ROS) production, UCP2 protein and manganese superoxide dismutase (Mn-SOD), in contrast to undifferentiated leukemia cells (M1). Mm1 cells expressed 10-fold more UCP2 protein compared with undifferentiated M1 cells, although the UCP2 mRNA levels in both cell types were similar. The higher expression of UCP2 in the Mm1 cells suggests a regulatory role of UCP2 in the ROS production. Furthermore, the transfection of UCP2-GFP-expression vector in Mm1 cells dissipated the mitochondrial membrane potential and reduced ROS production, which was shown by their direct visualization using MitoTracker Red CM-H(2)Xros. The macrophage gp91phox protein, a membrane catalytic component of the NADPH oxidase complex, was at a similar level in both of UCP2-GFP expressed and non-expressed Mm1 cells. These results suggest that the UCP2 protein of the undifferentiated cell is regulated at a quite low level and the higher UCP2 protein of the differentiated macrophages involves with the regulation of ROS production.