Screening and identification of salt-tolerance genes in Sophora alopecuroides and functional verification of SaAQP
Screening and identification of salt-tolerance genes in Sophora alopecuroides and functional verification of SaAQP
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DOI：
10.1007/s00425-021-03726-w
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发表时间：
2021-09
期刊：
影响因子：
4.3
通讯作者：
Youcheng Zhu;Qingyu Wang;Wenyun Guo;Ziwei Gao;Ying Wang;Yang Xu;Yajing Liu;Zhipeng Ma
中科院分区：
文献类型：
--
作者：
Youcheng Zhu;Qingyu Wang;Wenyun Guo;Ziwei Gao;Ying Wang;Yang Xu;Yajing Liu;Zhipeng Ma
结论过表达saaqp可提高转基因大豆毛状根的耐盐性。芥。摘要盐胁迫严重影响作物产量和粮食安全。提高作物的耐盐性是必要的，但耐盐基因的发现和利用仍然有限。苦豆子具有较强的抗逆性，是鉴定耐盐基因的理想材料。因此，我们旨在筛选和鉴定耐盐基因inS。苦豆子。利用酵母幼苗表达文库，利用含盐培养基模拟盐胁迫，筛选耐盐基因。通过盐处理筛选和转录组测序相结合，鉴定出11个与耐盐性相关的候选基因，包括过氧化物酶、肌醇甲基转移酶、水通道蛋白、半胱氨酸合成酶、果胶酯酶和WRKY基因。分析了盐处理后候选基因的表达动态。酵母BY4743的耐盐性得到了验证。候选基因参与了盐胁迫反应inS。它们的过表达显著提高了酵母的耐盐性。进一步在大豆毛状根和拟南芥中验证了saaqp介导的耐盐性，发现saaqp可能增强了a的耐盐性。蓝藻参与活性氧清除机制。该结果为植物抗盐育种提供了新的遗传资源。
Main conclusionOverexpression ofSaAQPcan improve the salt tolerance of transgenic soybean hairy roots andA. thaliana.AbstractSalt stress severely affects crop yield and food security. There is a need to improve the salt tolerance of crops, but the discovery and utilization of salt-tolerance genes remains limited. Owing to its strong stress tolerance,Sophora alopecuroidesis ideal for the identification of salt-tolerance genes. Therefore, we aimed to screen and identify the salt-tolerance genes inS. alopecuroides. With a yeast expression library of seedlings, salt-tolerant genes were screened using a salt-containing medium to simulate salt stress. By combining salt-treatment screening and transcriptome sequencing, 11 candidate genes related to salt tolerance were identified, including genes for peroxidase, inositol methyltransferase, aquaporin, cysteine synthase, pectinesterase, and WRKY. The expression dynamics of candidate genes were analyzed after salt treatment ofS. alopecuroides, and salt tolerance was verified in yeast BY4743. The candidate genes participated in the salt-stress response inS. alopecuroides, and their overexpression significantly improved the salt tolerance of yeast. Salt tolerance mediated bySaAQPwas further verified in soybean hairy roots andArabidopsis thaliana, and it was found thatSaAQPmight enhance the salt tolerance ofA. thalianaby participating in a reactive oxygen species scavenging mechanism. This result provides new genetic resources in plant breeding for salt resistance.