The influence of homologous vs. heterologous challenge virus strains on the serological test results of rabies virus neutralizing assays
The influence of homologous vs. heterologous challenge virus strains on the serological test results of rabies virus neutralizing assays
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DOI：
10.1016/j.biologicals.2005.06.005
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发表时间：
2005-12-01
期刊：
影响因子：
1.7
通讯作者：
Briggs, DJ
中科院分区：
文献类型：
--
作者：
Moore, SM;Ricke, TA;Briggs, DJ
评价了用于生产狂犬病疫苗的病毒种子株与用于测定接种后狂犬病病毒中和抗体的攻毒病毒株的相关性的影响。使用同源和异源检测系统，通过RFFIT在平行试验中检测了接种纯化Vero细胞狂犬病疫苗（PVRV）（由狂犬病病毒的Pittman摩尔（PM）种子株生产）或纯化鸡胚细胞狂犬病疫苗（PCECV）（由狂犬病病毒的Flury低代卵（Flury-LEP）种子株生产）的173例受试者的血清样本。在同源系统中，CVS-11用作试验中的攻毒病毒，以评价接种PVRV的受试者的体液免疫应答，Flury-LEP用于接种PCECV的受试者。在异源系统中，CVS-11用作试验中的攻毒病毒，以评价接种PCECV的受试者，Flury-LEP用于接种PVRV的受试者。尽管CVS-11和Flury-LEP狂犬病病毒株之间的G蛋白同源性差异据报道仅为5.8%，但与异源检测系统相比，使用同源检测系统导致两个疫苗组近三分之二样本的滴度高出约30%。两种不同疫苗接种后免疫应答等效性的评价取决于试验系统的类型，同源或异源。用于评价狂犬病毒中和抗体水平。当使用同源检测系统时，实现了疫苗之间的等效性，但当使用异源检测系统时，未实现。本研究的结果表明，用于测定接种后狂犬病病毒中和抗体水平的生物学试验的病毒株可能会深刻影响狂犬病疫苗的评价。(c)2005年，国际生物制品协会。由爱思唯尔有限公司出版。保留所有权利。
The effect that the relatedness of the viral seed strain used to produce rabies vaccines has to the strain of challenge virus used to measure rabies virus neutralizing antibodies after vaccination was evaluated. Serum samples from 173 subjects vaccinated with either purified Vero cell rabies vaccine (PVRV), produced from the Pittman Moore (PM) seed strain of rabies virus, or purified chick embryo cell rabies vaccine (PCECV), produced from the Flury low egg passage (Flury-LEP) seed strain of rabies virus, were tested in parallel assays by RFFIT using a homologous and a heterologous testing system. In the homologous system, CVS-11 was used as the challenge virus in the assay to evaluate the humoral immune response in subjects vaccinated with PVRV and Flury-LEP was used for subjects vaccinated with PCECV. In the heterologous system, CVS-11 was used as the challenge virus in the assay to evaluate subjects vaccinated with PCECV and Flury-LEP was used for subjects vaccinated with PVRV. Although the difference in G protein homology between the CVS-11 and Flury-LEP rabies virus strains has been reported to be only 5.8%, the use of a homologous testing system resulted in approximately 30% higher titers for nearly two-thirds of the samples from both vaccine groups compared to a heterologous testing system. The evaluation of equivalence of the immune response after vaccination with the two different vaccines was dependent upon the type of testing system, homologous or heterologous. used to evaluate the level of rabies virus neutralizing antibodies. Equivalence between the vaccines was achieved when a homologous testing system was used but not when a heterologous testing system was used. The results of this study indicate that the strain of virus used in the biological assays to measure the level of rabies virus neutralizing antibodies after vaccination could profoundly influence the evaluation of rabies vaccines. (c) 2005 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.