Blood Viscosity and the Expression of Inflammatory and Adhesion Markers in Homozygous Sickle Cell Disease Subjects with Chronic Leg Ulcers
Blood Viscosity and the Expression of Inflammatory and Adhesion Markers in Homozygous Sickle Cell Disease Subjects with Chronic Leg Ulcers
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DOI：
10.1371/journal.pone.0068929
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发表时间：
2013-07-26
期刊：
影响因子：
3.7
通讯作者：
Reid, Marvin
中科院分区：
文献类型：
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作者：
Bowers, Andre S.;Reid, Harvey L.;Reid, Marvin
目的：测定纯合子S型镰状细胞病（SCD）伴和不伴慢性腿部溃疡者与AA基因型对照者在46秒（-1）和230秒（-1）剪切速率下tnf - α、IL-1 β、IL-10、sICAM-1浓度、腿部缺氧和全血粘度（WBV）的差异。设计与方法：55名年龄匹配的参与者被招募到研究中：31名没有腿部溃疡的SS受试者（SSn）， 24名腿部溃疡的SS受试者（SSu）和18名AA对照组。血液学指标采用AC. Tron Coulter计数器测量。采用ELISA法对炎症、抗炎和粘附分子进行定量分析。全血粘度测量使用Wells Brookfield锥形板粘度计完成。用可见光导分光光度法定量微血管组织氧合。结果：46秒（-1）和230秒（-1）时tnf - α和全血粘度(1.75,2.02 vs. 0.83, 1.26, p
Objective: To determine differences in TNF-alpha, IL-1 beta, IL-10, sICAM-1 concentrations, leg hypoxia and whole blood viscosity (WBV) at shear rates of 46 sec(-1) and 230 sec(-1) in persons with homozygous S sickle cell disease (SCD) with and without chronic leg ulceration and in AA genotype controls.Design: & Methods: fifty-five age-matched participants were recruited into the study: 31 SS subjects without leg ulcers (SSn), 24 SS subjects with leg ulcers (SSu) and 18 AA controls. Haematological indices were measured using an AC. Tron Coulter Counter. Quantification of inflammatory, anti-inflammatory and adhesion molecules was performed by ELISA. Measurement of whole blood viscosity was done using a Wells Brookfield cone-plate viscometer. Quantification of microvascular tissue oxygenation was done by Visible Lightguide spectrophotometry.Results: TNF-alpha and whole blood viscosity at 46 sec(-1) and 230 sec(-1) (1.75, 2.02 vs. 0.83, 1.26, p