Activation of a Rac GTPase by the NLR Family Disease Resistance Protein Pit Plays a Critical Role in Rice Innate Immunity
Activation of a Rac GTPase by the NLR Family Disease Resistance Protein Pit Plays a Critical Role in Rice Innate Immunity
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DOI：
10.1016/j.chom.2010.04.010
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发表时间：
2010-05-20
影响因子：
30.3
通讯作者：
Shimamoto, Ko
中科院分区：
文献类型：
--
作者：
Kawano, Yoji;Akamatsu, Akira;Shimamoto, Ko
核苷酸结合结构域和富含亮氨酸的重复序列（NLR）家族蛋白识别病原体衍生的分子，并触发植物和动物的免疫反应。在植物中，NLR对特定病原体效应物的直接或间接识别最终导致过敏反应（HR）和活性氧（ROS）的产生，这是植物防御反应的关键组成部分。然而，NLR激活的分子以及它们如何诱导免疫反应在很大程度上是未知的。我们发现，在质膜上的水稻GTbaseOs-Rac 1直接与Pit相互作用，Pit是一种NLR蛋白，赋予对稻瘟病菌的抗性。OsRac 1参与Pit介导的ROS产生以及HR，并且是Pit介导的水稻抗病性所必需的。此外，Pit的活性形式诱导质膜上OsRac 1的活化。因此，OsRac 1在病原菌侵染过程中被Pit激活，在Pit介导的水稻免疫中发挥重要作用。
The nucleotide-binding domain and leucine-rich repeat-containing (NLR) family proteins recognize pathogen-derived molecules and trigger immune responses in both plants and animals. In plants, the direct or indirect recognition of specific pathogen effectors by NLRs culminates in a hypersensitive response (HR) and the production of reactive oxygen species (ROS), key components of the plant defense response. However, the molecules activated by NLRs and how they induce immune responses are largely unknown. We found that the rice GTPase Os-Rac1 at the plasma membrane interacts directly with Pit, an NLR protein that confers resistance to the rice blast fungus. OsRac1 contributes to Pit-mediated ROS production as well as the HR and is required for Pit-mediated disease resistance in rice. Furthermore, the active form of Pit induces the activation of OsRac1 at the plasma membrane. Thus, OsRac1 is activated by Pit during pathogen attack and plays a critical role in Pit-mediated immunity in rice.