KAHWEOL AND CAFESTOL - INHIBITORS OF HAMSTER BUCCAL POUCH CARCINOGENESIS
KAHWEOL AND CAFESTOL - INHIBITORS OF HAMSTER BUCCAL POUCH CARCINOGENESIS
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DOI：
10.1080/01635589109514110
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发表时间：
1991-01-01
影响因子：
2.9
通讯作者：
SUNAHARA, GI
中科院分区：
文献类型：
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作者：
MILLER, EG;MCWHORTER, K;SUNAHARA, GI
从生咖啡豆中提取的两种化合物Kahweol和cafestol被用于癌症化学预防活性的测试。在实验中，60只仓鼠被分成三组，分别吃三种食物中的一种。ⅰ组饲喂正常饲粮，ⅱ组和ⅲ组在相同饲粮中添加50:50的咖啡豆醇和咖啡醇混合物。两种饲粮中咖啡豆醇和咖啡醇混合物的含量分别为0.2 g/kg食物（第二组）和2.0 g/kg食物（第三组）。在仓鼠适应各自的饮食后，从每组中选择16只仓鼠。这些动物的左颊袋每周用0.5%的7,12-二甲基苯[a]蒽（DMBA）矿物油溶液涂三次。其余12只仓鼠作为对照。这些动物的左颊袋每周用矿物油涂三次。13周后（39次），仓鼠被杀死。用DMBA治疗的动物多发肿瘤；然而，在饮食中摄入咖啡豆醇和咖啡醇（每公斤食物2克）的动物的肿瘤负担减少了35%。I组和III组之间的进一步比较表明，肿瘤负荷的减少是由于肿瘤数量的减少。第二组的结果尚无定论。发现肿瘤数量有所减少，但这被肿瘤大小的增加所抵消。
Kahweol and cafestol, two compounds extracted from green coffee beans, were tested for cancer chemopreventive activity. For the experiment, 60 hamsters were divided into three equal groups and placed on one of three diets. The animals in Group I received a normal diet, whereas the animals in Groups II and III received the same diet supplemented with a 50:50 mixture of kahweol and cafestol. The content of the kahweol and cafestol mixture in these two diets was 0.2 g/kg of food (Group II) and 2.0 g/kg of food (Group III). After the hamsters adjusted to their respective diets, 16 hamsters from each group were selected. The left buccal pouches of these animals were painted three times weekly with a 0.5% solution of 7,12-dimethylbenz[a]anthracene (DMBA) in mineral oil. The 12 remaining hamsters were used as controls. The left buccal pouches of these animals were painted three times weekly with mineral oil. After 13 weeks (39 applications) the hamsters were killed. Multiple tumors were common in animals treated with DMBA; however, the animals receiving kahweol and cafestol in the diet (2 g/kg of food) exhibited a 35% reduction in tumor burden. Further comparisons between Groups I and III showed that this reduction in tumor burden was due to a decrease in tumor number. The results for Group II were inconclusive. Some reduction in tumor number was found, but this was offset by an increase in the size of the tumors.