EXTRACELLULAR-MATRIX GENE-EXPRESSION BY HUMAN BONE-MARROW STROMA AND BY MARROW FIBROBLASTS
EXTRACELLULAR-MATRIX GENE-EXPRESSION BY HUMAN BONE-MARROW STROMA AND BY MARROW FIBROBLASTS
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DOI：
10.3109/15419069309095685
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
MINGUELL, JJ
中科院分区：
文献类型：
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作者：
CHICHESTER, CO;FERNANDEZ, M;MINGUELL, JJ
在骨髓空间中，造血祖细胞与基质细胞相互作用，允许归巢、迁移和表达发育程序。其中一些相互作用是由骨髓基质细胞产生的细胞外基质分子（ECM）介导的。在小鼠系统中，这些相互作用的性质及其生物学意义已在体外研究，使用不清楚，无论是整个基质（从长期骨髓培养）或分离的基质（成纤维细胞样）细胞。为了分析在人类系统中两种来源的基质细胞在ECM组分的产生方面是否等同，我们测量了编码几种ECM蛋白的基因的表达。所获得的结果表明，整个基质以及骨髓成纤维细胞表达I、III、IV、V和VI型胶原蛋白以及纤连蛋白和层粘连蛋白的基因。然而，与整个基质相比，骨髓成纤维细胞中这些蛋白质的mRNA转录物的相对丰度更高。与整个基质相比，在骨髓成纤维细胞中观察到的胶原蛋白合成增加进一步支持了后者。
In the marrow space hemopoietic progenitors interact with stromal cells allowing homing, migration and the expression of developmental programs. Some of these interactions are mediated by extracellular matrix molecules (ECM) which are produced by marrow stromal cells. In the murine system the nature of these interactions and its biological significance have been studied in vitro by using indistinctly, either whole stroma (obtained from long-term marrow cultures) or isolated stromal (fibroblast-like) cells. In an attempt to analyze whether in the human system both sources of stromal cells are equivalent in terms of production of ECM components, we measured the expression of genes encoding for several ECM proteins. The results obtained show that whole stroma as well as marrow fibroblasts express genes for collagen types I, III, IV, V and VI as well as for fibronectin and laminin. However, the relative abundance of mRNA transcripts for some of these proteins was higher in marrow fibroblasts as compared to whole stroma. The latter was further supported by an increased collagen synthesis observed in marrow fibroblasts as compared to whole stroma.