High bacterial loads of Ureaplasma may be associated with non-specific cervicitis
High bacterial loads of Ureaplasma may be associated with non-specific cervicitis
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DOI：
10.3109/00365548.2014.922696
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发表时间：
2014-09-01
影响因子：
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通讯作者：
Huang, Yanqun
中科院分区：
文献类型：
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作者：
Liu, Lu;Cao, Guojun;Huang, Yanqun
背景资料：微小脲原体和解脲脲原体常见于非衣原体和非淋菌性宫颈炎或非特异性宫颈炎（NSC）妇女的宫颈。然而，他们的贡献NSC的病因是有争议的。方法：美国U. parvum和U.使用实时PCR，在从155名NSC妇女和312名无NSC对照妇女收集的宫颈拭子中鉴定和定量解脲支原体。然后使用脲原体拷贝数除以宿主细胞数计算相对细菌定量;这对于校正与不同拭子中收获的细胞数相关的偏倚非常重要。结果：155例NSC患者中有91例（58.7%）检出支原体; parvum占30.3%，U.解脲支原体感染占16.1%，混合感染占12.3%。在对照组中也有54.5%（170/312）检出：U. parvum占33.0%，U.解脲支原体感染占11.5%，混合感染占9.9%。两组间无显著性差异。parvum、小盾壳霉U.两组间解脲支原体感染或混合感染差异无统计学意义（P > 0.05）。然而，两种生物型在NSC患者中的浓度均高于对照组（p < 0.05）。以> 10拷贝/1000细胞为参照，U. NSC患者U. p. parvum阳性率为16.1%，显著高于对照组的5.1%（相对危险度3.145，p < 0.05）; NSC患者和对照组的解脲支原体感染率分别为28.4%和8.7%，差异有统计学意义（相对危险度3.131，p < 0.05）。结论：脲原体可粘附于宿主细胞，定植，内化，并随后产生病理损害。宫颈内高密度的脲原体可能与NSC的病因有关。
Background: Ureaplasma parvum and Ureaplasma urealyticum are commonly found in the cervix of women with non-chlamydial and non-gonococcal cervicitis or non-specific cervicitis (NSC). However their contribution to the aetiology of NSC is controversial. Methods: U. parvum and U. urealyticum were identified and quantified in cervical swabs collected from 155 women with NSC and 312 controls without NSC, using real-time PCR. The relative bacterial quantification was then calculated using the Ureaplasma copy number divided by the number of host cells; this is important for the correction of bias linked to the number of cells harvested in different swabs. Results: Ureaplasma was detected in 58.7% (91/155) of NSC patients: U. parvum in 30.3%, U. urealyticum in 16.1%, and mixed infection in 12.3%. It was also detected in 54.5% (170/312) of controls: U. parvum in 33.0%, U. urealyticum in 11.5%, and mixed infection in 9.9%. There were no significant differences for U. parvum, U. urealyticum, or mixed infection between the 2 groups (p > 0.05). However, both biovars were present at higher concentrations in NSC patients than in controls (p < 0.05). Using > 10 copies/1000 cells as a reference, the positive rate of U. parvum in NSC patients was 16.1%, significantly higher than that in controls at 5.1% (relative risk 3.145, p < 0.05); positive rates of U. urealyticum in NSC patients and controls were 28.4% and 8.7%, respectively, with a statistically significant difference (relative risk 3.131, p < 0.05). Conclusions: Ureaplasma can adhere to host cells, colonize, internalize, and subsequently produce pathological lesions. A high density of Ureaplasma in the cervix may be associated with the aetiology of NSC.