Fat extract improves fat graft survival via proangiogenic, anti-apoptotic and pro-proliferative activities
Fat extract improves fat graft survival via proangiogenic, anti-apoptotic and pro-proliferative activities
复制标题
脂肪提取物通过促血管生成、抗凋亡和促增殖活性提高脂肪移植物的存活率
DOI：
10.1186/s13287-019-1290-1
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发表时间：
2019-06-13
影响因子：
7.5
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Zheng, Hongjie;Yu, Ziyou;Li, Wei
背景我们先前的研究证明纳米脂肪可以通过促进新生血管形成来提高脂肪移植物的存活率。脂肪提取物（FE），来自nanofat的无细胞成分，也具有proangiogenic activity.ObjectivesThe目的本研究是调查是否FE可以提高脂肪移植物的存活率和FE和nanofat是否可以协同工作，以促进脂肪移植物的存活。潜在的机制也investigated.MethodsIn第一次动物研究中，从脂肪抽吸的人巨脂肪与FE或nanofat共移植到裸鼠体内。分别于术后2、4、12周对移植物进行评价。在第二个动物研究中，裸鼠移植了macrofat和nanofat的混合物，然后在移植后第1、7、14、21和28天移植物内注射FE。移植后12周对移植物进行评价。为了检测FE影响移植物存活的机制，FE的促血管生成、抗凋亡和促增殖活性在体内移植物和体外培养的人血管内皮细胞（HUVECs）、脂肪源性干细胞（ADSC）和脂肪组织中进行了分析。纳米脂肪和FE处理组中脂肪移植物的重量显著高于对照组中脂肪移植物的重量。此外，在纳米脂肪和FE处理组中观察到更高的脂肪完整性，更多的活脂肪细胞，更多的CD31阳性血管，更少的凋亡细胞和更多的Ki67阳性增殖细胞。在第二项动物研究中，纳米脂肪+FE组中脂肪移植物的重量显著高于对照组中脂肪移植物的重量。在体外，FE显示促血管生成的HUVECs，抗凋亡的脂肪组织在缺氧条件下培养的效果和能力，以促进ADSC增殖，并保持其多分化capital.ConclusionsFE可以提高脂肪移植物的存活率通过促血管生成，抗凋亡和促ADSC增殖的影响。FE加纳米脂肪辅助脂肪移植是一种新的策略，可能会在临床应用中使用。
BackgroundOur previous study proved that nanofat could enhance fat graft survival by promoting neovascularization. Fat extract (FE), a cell-free component derived from nanofat, also possesses proangiogenic activity.ObjectivesThe aim of this study was to investigate whether FE could improve fat graft survival and whether FE and nanofat could work synergistically to promote fat graft survival. The underlying mechanism was also investigated.MethodsIn the first animal study, human macrofat from lipoaspirate was co-transplanted into nude mice with FE or nanofat. The grafts were evaluated at 2, 4 and 12weeks post-transplantation. In the second animal study, nude mice were transplanted with a mixture of macrofat and nanofat, followed by intra-graft injection of FE at days 1, 7, 14, 21 and 28 post-transplantation. The grafts were evaluated at 12weeks post-transplantation. To detect the mechanism by which FE impacts graft survival, the proangiogenic, anti-apoptotic and pro-proliferative activities of FE were analysed in grafts in vivo and in cultured human vascular endothelial cells (HUVECs), adipose-derived stem cells (ADSCs) and fat tissue in vitro.ResultsIn the first animal study, the weights of the fat grafts in the nanofat- and FE-treated groups were significantly higher than those of the fat grafts in the control group. In addition, higher fat integrity, more viable adipocytes, more CD31-positive blood vessels, fewer apoptotic cells and more Ki67-positive proliferating cells were observed in the nanofat- and FE-treated groups. In the second animal study, the weights of the fat grafts in the nanofat+FE group were significantly higher than those of the fat grafts in the control group. In vitro, FE showed proangiogenic effects on HUVECs, anti-apoptotic effects on fat tissue cultured under hypoxic conditions and an ability to promote ADSC proliferation and maintain their multiple differentiation capacity.ConclusionsFE could improve fat graft survival via proangiogenic, anti-apoptotic and pro-proliferative effects on ADSCs. FE plus nanofat-assisted fat grafting is a new strategy that could potentially be used in clinical applications.