Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP
Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP
复制标题
DOI：
10.1016/s0896-6273(00)80044-6
复制
发表时间：
1996-02-01
期刊：
影响因子：
16.2
通讯作者：
Nakanishi, S
中科院分区：
文献类型：
--
作者：
Moriyoshi, K;Richards, LJ;Nakanishi, S
我们描述了一种实验系统，通过使用重组腺病毒载体将水母绿色荧光蛋白(GFP)转移到体外和体内的有丝分裂后神经细胞中，显示活的和固定的准备中哺乳动物神经元和胶质细胞的胞体和突起。我们已经介绍了几种GFP的修饰，以增强其在哺乳动物轴突和树突中的荧光强度。该方法可用于研究细胞迁移和神经元连接的动态过程，以及用腺病毒载体分析外源基因导入细胞的功能。
We describe an experimental system to visualize the soma and processes of mammalian neurons and glia in living and fixed preparations by using a recombinant adenovirus vector to transfer the jellyfish green fluorescent protein (GFP) into postmitotic neural cells both in vitro and in vivo. We have introduced several modifications of GFP that enhance its fluorescence intensity in mammalian axons and dendrites. This method should be useful for studying the dynamic processes of cell migration and the development of neuronal connections, as well as for analyzing the function of exogenous genes introduced into cells using the adenovirus vector.