DEVELOPMENT OF FIBROBLAST COLONIES IN MONOLAYER CULTURES OF GUINEA-PIG BONE MARROW AND SPLEEN CELLS
DEVELOPMENT OF FIBROBLAST COLONIES IN MONOLAYER CULTURES OF GUINEA-PIG BONE MARROW AND SPLEEN CELLS
复制标题
DOI：
10.1111/j.1365-2184.1970.tb00347.x
复制
发表时间：
1970-01-01
期刊：
影响因子：
--
通讯作者：
LALYKINA, KS
中科院分区：
文献类型：
--
作者：
FRIEDENSTEIN, AJ;CHAILAKHJAN, RK;LALYKINA, KS
在豚鼠骨髓和脾脏的单层培养物中，离散成纤维细胞集落的形成发生在第9-12天。随着成纤维细胞数量的增加，集落数量呈线性增加，混合培养物中雄性和雌性细胞的分布支持成纤维细胞集落是克隆的观点。骨髓和脾脏中集落形成细胞的浓度约为10 - 5。骨髓培养（但不是脾培养）成纤维细胞能够在扩散室中自发形成骨。在移行上皮存在的情况下，来自骨髓和脾培养物的成纤维细胞在扩散室中可诱导成骨。
In monolayer cultures of guinea‐pig bone marrow and spleen the development of discrete fibroblast colonies takes place on days 9–12. The linear increase in the number of colonies with increasing numbers of explanted cells and the distribution of male and female cells in mixed cultures support the view that fibroblast colonies are clones. The concentration of colony‐forming cells in bone marrow and spleen is approximately 10‐5. Bone marrow culture (but not spleen culture) fibroblasts are capable of spontaneous bone formation in diffusion chambers. Fibroblasts from both bone marrow and spleen cultures are inducible to osteogenesis in diffusion chambers in the presence of transitional epithelium.